NME1 / NDKA Antibody ( FITC )

NME1 / NDKA Antibody Datasheet

NME1 / NDKA Antibody Product Information

• Immunogen: Recombinant Human NME1 / NDKA protein (Catalog#11615-H07E)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 01
• Ig Type: Mouse IgG1
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NME1 / NDKA (rh NME1 / NDKA; Catalog#11615-H07E; NP_000260.1; Ala 2-Glu 152) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

NME1 / NDKA Antibody Usage Guide

• Specificity: Human NME1 / NDKA
• Flow Cytometry:
  

  Flow cytometric analysis of Human NME1 expression in HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-NME1 (11615-MM01-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

NME1 / NDKA Antibody Background

Nucleoside diphosphate kinase A, also known as NDP kinase A, Tumor metastatic process-associated protein, Metastasis inhibition factor nm23, Granzyme A-activated Dnase, GAAD, NME1 and NDPKA, is a cytoplasm protein which belongs to the NDK family. Isoform 1 of NME1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 NME1 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 NME1 is ubiquitously expressed. NME1 / NDPKA is found in reduced amount in some tumor cells of high metastatic potential. However, increased NME1 levels correlate with aggressive tumor features in neuroblastoma. NME1 / NDPKA may have distinct if not opposite roles in different tumors. NME1 / NDPKA possesses nucleoside-diphosphate kinase, serine / threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. It is involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. NME1 / NDPKA is required for neural development including neural patterning and cell fate determination.

References

1. Hailat N. et al., 1991, J. Clin. Invest. 88:341-5.
2. MacDonald N.J. et al., 1996, J. Biol. Chem. 271: 25107-16.
3. Fan Z. et al., 2003, Cell 112: 659-72.
4. Dephoure N. et al., 2008, Proc. Natl. Acad. Sci. USA. 105:10762-7.
5. Choudhary C. et al., 2009, Science. 325: 834-40.