Human NMB Gene cDNA Clone / ORF Clone

NMB cDNA Clone Datasheet

NMB cDNA Clone Product Information

• Gene Bank Ref.ID: BC007407
• cDNA Size: 465
• Gene Synonym: NMB
• Source: Human

NMB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens neuromedin B DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NMB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NMB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NMB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCGGC GGGCGGGGGG CGCTCGGATG TTCGGCAGCC TCCTGCTCTT CGCCCTGCTC
61       GCTGCCGGCG TCGCCCCGCT CAGCTGGGAT CTCCCGGAGC CCCGCAGCCG AGCCAGCAAG
121      ATCCGAGTGC ACTCGCGAGG CAACCTCTGG GCCACCGGTC ACTTCATGGG CAAGAAGAGT
181      CTGGAGCCTT CCAGCCCATC CCCATTGGGG ACAGCTACCC ACACCTCCCT GAGGGACCAG
241      CGACTGCAGC TGAGTCATGA TCTGCTCGGA ATCCTCCTGC TAAAGAAGGC TCTGGGCGTG
301      AGCCTCAGCC GCCCCGCACC CCAAATCCAG GAGGCTGCTG GTACAAATAC TGCAGAAATG
361      ACACCAATAA TGGGGCAGAC ACAACAGCGT GGCTTAGATT GTGCCCACCC AGGGAAGGTG
421      CTGAATGGGA CCCTGTTGAT GGCCCCATCT GGATGTAAAT CCTGA

 

Quality Control:cDNA sequencing results is available upon request.