NKG2A / CD159A / KLRC1 Protein (His Tag)

Killer cell lectin-like receptor subfamily C, member 1 Protein Datasheet

NKG2A / CD159A / KLRC1 Protein Product Information

• Synonym: KLRC1, NKG2A
• Protein Construction: A DNA sequence encoding the mature form of human KLRC1(NP_002250) (Arg100-Leu233) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Human Cells

NKG2A / CD159A / KLRC1 Protein QC Testing

• Purity: > 93 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human human KLRC1 consists of 154 amino acids and predicts a molecular mass of 17.8 KDa. It migrates as an approximately 30-40 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NKG2A / CD159A / KLRC1 protein

NKG2A / CD159A / KLRC1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NKG2A / CD159A / KLRC1 Protein Description

NKG2, also known as NKG2A(CD159A), contains 1 C-type lectin domain and belongs to the killer cell lectin-like receptor family. The killer cell lectin-like receptor family is a group of transmembrane proteins preferentially expressed in NK cells. Members of this proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. NKG2 is expressed only in NK-cells, but not in T-cells or B-cells. It has been shown that NKG2 represents a family of related cDNA clones, designated NKG2A, NKG2B, NKG2C, and NKG2D, which encode type 2 integral membrane proteins (extracellular C-terminus) containing a C-type lectin domain. NKG2 forms a complex with another family member, KLRD1/CD94. NKG2A and NKG2B appear to be splice variants of the same gene and have been given the designation CD159a in the nomenclature of CD antigens. NKG2 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.

References

1. Angelini DF et al., 2011, J Leukoc Biol. 89(1):75-84.
2. Ge SJ et al., 2011, Zhongguo Shi Yan Xue Ye Xue Za Zhi. 19(2):312-6.
3. Ablamunits V et al., 2011, Eur J Immunol. 41(7):1832-42.