Human NIT1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13747-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NIT1 cDNA Clone Datasheet

NIT1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC046149
cDNA Size:	732
Gene Synonym:	MGC57670; NIT1
Source:	Human

NIT1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens nitrilase 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

NIT1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NIT1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NIT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCCTTCTGTG TCCTGGACTC
61       CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC
121      TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG
181      CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTCCCTGC
241      CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC
301      CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT
361      GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG
421      AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAAAGGGG CAGTAGTGGC CACTTACAGG
481      AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT
541      ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA
601      GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA
661      GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCATTACAG GCCCAGCCCA CTGGGAGCCC
721      GTCTCCTCTT GA

Quality Control:cDNA sequencing results is available upon request.