Human NGFR ORF Expression Clone, untagged (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13184-G-N	pCMV / hygro ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NGFR cDNA Clone Datasheet

NGFR cDNA Clone Product Information

Gene Bank Ref.ID:	NM_002507.2
cDNA Size:	1284
Gene Synonym:	CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; NGFR
Source:	Human

NGFR cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens nerve growth factor receptor (TNFR superfamily, member 16) DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 6 G/A not causing the amino acid variation.

NGFR cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NGFR cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NGFR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGCAG GTGCCACCGG CCGCGCCATG GACGGGCCGC GCCTGCTGCT GTTGCTGCTT
61       CTGGGGGTGT CCCTTGGAGG TGCCAAGGAG GCATGCCCCA CAGGCCTGTA CACACACAGC
121      GGTGAGTGCT GCAAAGCCTG CAACCTGGGC GAGGGTGTGG CCCAGCCTTG TGGAGCCAAC
181      CAGACCGTGT GTGAGCCCTG CCTGGACAGC GTGACGTTCT CCGACGTGGT GAGCGCGACC
241      GAGCCGTGCA AGCCGTGCAC CGAGTGCGTG GGGCTCCAGA GCATGTCGGC GCCGTGCGTG
301      GAGGCCGACG ACGCCGTGTG CCGCTGCGCC TACGGCTACT ACCAGGATGA GACGACTGGG
361      CGCTGCGAGG CGTGCCGCGT GTGCGAGGCG GGCTCGGGCC TCGTGTTCTC CTGCCAGGAC
421      AAGCAGAACA CCGTGTGCGA GGAGTGCCCC GACGGCACGT ATTCCGACGA GGCCAACCAC
481      GTGGACCCGT GCCTGCCCTG CACCGTGTGC GAGGACACCG AGCGCCAGCT CCGCGAGTGC
541      ACACGCTGGG CCGACGCCGA GTGCGAGGAG ATCCCTGGCC GTTGGATTAC ACGGTCCACA
601      CCCCCAGAGG GCTCGGACAG CACAGCCCCC AGCACCCAGG AGCCTGAGGC ACCTCCAGAA
661      CAAGACCTCA TAGCCAGCAC GGTGGCAGGT GTGGTGACCA CAGTGATGGG CAGCTCCCAG
721      CCCGTGGTGA CCCGAGGCAC CACCGACAAC CTCATCCCTG TCTATTGCTC CATCCTGGCT
781      GCTGTGGTTG TGGGCCTTGT GGCCTACATA GCCTTCAAGA GGTGGAACAG CTGCAAGCAG
841      AACAAGCAAG GAGCCAACAG CCGGCCAGTG AACCAGACGC CCCCACCAGA GGGAGAAAAA
901      CTCCACAGCG ACAGTGGCAT CTCCGTGGAC AGCCAGAGCC TGCATGACCA GCAGCCCCAC
961      ACGCAGACAG CCTCGGGCCA GGCCCTCAAG GGTGACGGAG GCCTCTACAG CAGCCTGCCC
1021     CCAGCCAAGC GGGAGGAGGT GGAGAAGCTT CTCAACGGCT CTGCGGGGGA CACCTGGCGG
1081     CACCTGGCGG GCGAGCTGGG CTACCAGCCC GAGCACATAG ACTCCTTTAC CCATGAGGCC
1141     TGCCCCGTTC GCGCCCTGCT TGCAAGCTGG GCCACCCAGG ACAGCGCCAC ACTGGACGCC
1201     CTCCTGGCCG CCCTGCGCCG CATCCAGCGA GCCGACCTCG TGGAGAGTCT GTGCAGTGAG
1261     TCCACTGCCA CATCCCCGGT GTAA

Quality Control:cDNA sequencing results is available upon request.

Cytokine