Human NGFR Gene cDNA Clone / ORF Clone (Cytokine)

NGFR cDNA Clone Datasheet

NGFR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002507.2
• cDNA Size: 1284
• Gene Synonym: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; NGFR
• Source: Human

NGFR cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens nerve growth factor receptor (TNFR superfamily, member 16) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 6 G/A not causing the amino acid variation.

NGFR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NGFR cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NGFR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGCAG GTGCCACCGG CCGCGCCATG GACGGGCCGC GCCTGCTGCT GTTGCTGCTT
61       CTGGGGGTGT CCCTTGGAGG TGCCAAGGAG GCATGCCCCA CAGGCCTGTA CACACACAGC
121      GGTGAGTGCT GCAAAGCCTG CAACCTGGGC GAGGGTGTGG CCCAGCCTTG TGGAGCCAAC
181      CAGACCGTGT GTGAGCCCTG CCTGGACAGC GTGACGTTCT CCGACGTGGT GAGCGCGACC
241      GAGCCGTGCA AGCCGTGCAC CGAGTGCGTG GGGCTCCAGA GCATGTCGGC GCCGTGCGTG
301      GAGGCCGACG ACGCCGTGTG CCGCTGCGCC TACGGCTACT ACCAGGATGA GACGACTGGG
361      CGCTGCGAGG CGTGCCGCGT GTGCGAGGCG GGCTCGGGCC TCGTGTTCTC CTGCCAGGAC
421      AAGCAGAACA CCGTGTGCGA GGAGTGCCCC GACGGCACGT ATTCCGACGA GGCCAACCAC
481      GTGGACCCGT GCCTGCCCTG CACCGTGTGC GAGGACACCG AGCGCCAGCT CCGCGAGTGC
541      ACACGCTGGG CCGACGCCGA GTGCGAGGAG ATCCCTGGCC GTTGGATTAC ACGGTCCACA
601      CCCCCAGAGG GCTCGGACAG CACAGCCCCC AGCACCCAGG AGCCTGAGGC ACCTCCAGAA
661      CAAGACCTCA TAGCCAGCAC GGTGGCAGGT GTGGTGACCA CAGTGATGGG CAGCTCCCAG
721      CCCGTGGTGA CCCGAGGCAC CACCGACAAC CTCATCCCTG TCTATTGCTC CATCCTGGCT
781      GCTGTGGTTG TGGGCCTTGT GGCCTACATA GCCTTCAAGA GGTGGAACAG CTGCAAGCAG
841      AACAAGCAAG GAGCCAACAG CCGGCCAGTG AACCAGACGC CCCCACCAGA GGGAGAAAAA
901      CTCCACAGCG ACAGTGGCAT CTCCGTGGAC AGCCAGAGCC TGCATGACCA GCAGCCCCAC
961      ACGCAGACAG CCTCGGGCCA GGCCCTCAAG GGTGACGGAG GCCTCTACAG CAGCCTGCCC
1021     CCAGCCAAGC GGGAGGAGGT GGAGAAGCTT CTCAACGGCT CTGCGGGGGA CACCTGGCGG
1081     CACCTGGCGG GCGAGCTGGG CTACCAGCCC GAGCACATAG ACTCCTTTAC CCATGAGGCC
1141     TGCCCCGTTC GCGCCCTGCT TGCAAGCTGG GCCACCCAGG ACAGCGCCAC ACTGGACGCC
1201     CTCCTGGCCG CCCTGCGCCG CATCCAGCGA GCCGACCTCG TGGAGAGTCT GTGCAGTGAG
1261     TCCACTGCCA CATCCCCGGT GTGA

 

Quality Control:cDNA sequencing results is available upon request.

NGFR / P75 Gene Description

Nerve growth factor receptors(NGFRs or P75) include high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. High-affinity nerve growth factor receptor is also known as TrkA. Nerve growth factor binds with TrkA after being released from target cells, the NGF / TrkA complex is subsequently trafficked back to the cell body. NGFLow-affinity nerve growth factor receptor also named p75 neurotrophin receptor. All the four kinds of neurotrophins, including Nerve growth factor, Brain derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75NTR. It has been demonstrated that NGF acts through its low-affinity receptor p75NTR. Studies have proved that NGFR acts as a molecular signal swith that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

References

1. Chen LW. et al., 2008, CNS Neurol Disord Drug Targets. 7 (6): 512-23.
2. Deponti D. et al., 2009, Mol Biol Cell. 20 (16): 3620-7.
3. Ken-ichiro K. et al., 2004, J Biol Chem. 279 (3): 1703-12.