Mouse NGFR Gene cDNA Clone / ORF Clone

NGFR cDNA Clone Datasheet

NGFR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_033217.3
• cDNA Size: 1278
• Gene Synonym: p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
• Source: Mouse

NGFR cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus nerve growth factor receptor (TNFR superfamily, member 16) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 46-51 deletion mutation and 679 A/G resulting in the amino acid Ile substitution by Val，702 G/A not causing the amino acid variation.

NGFR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NGFR cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NGFR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGAGGG CAGGTGCTGC CTGCAGCGCC ATGGACCGGC TGCGCCTGCT GCTGCTGCTG
61       CTTCTAGGGG TGTCCTTTGG AGGTGCCAAG GAGACATGTT CCACAGGCAT GTACACCCAC
121      AGTGGAGAGT GCTGCAAAGC CTGCAACCTG GGCGAAGGTG TGGCCCAGCC TTGCGGAGCC
181      AACCAGACCG TGTGTGAACC CTGCCTGGAC AGTGTTACGT TCTCTGACGT GGTGAGCGCC
241      ACCGAGCCGT GCAAGCCGTG CACCGAGTGC CTGGGCCTGC AGAGTATGTC CGCTCCCTGT
301      GTGGAGGCAG ACGATGCCGT GTGCCGATGC TCCTATGGCT ACTACCAGGA CGAGGAGACT
361      GGCCGCTGCG AGGCTTGCAG CGTGTGCGGG GTGGGCTCAG GACTCGTGTT CTCCTGCCAG
421      GACAAACAGA ACACAGTGTG TGAAGAGTGC CCAGAGGGCA CATACTCAGA TGAAGCCAAC
481      CACGTGGACC CGTGCCTACC CTGCACGGTG TGCGAGGACA CTGAGCGCCA GTTACGCGAG
541      TGCACGCCCT GGGCTGACGC CGAATGCGAG GAGATCCCTG GCCGATGGAT CACAAGGTCT
601      ACGCCCCCGG AGGGCTCTGA CGTCACAACA CCCAGCACCC AGGAGCCGGA GGCACCTCCA
661      GAGCGAGACC TCGTAGCCAG CACAGTGGCC GATACAGTGA CCACTGTGAT GGGCAGCTCC
721      CAGCCTGTAG TGACCCGAGG CACCGCTGAC AACCTCATTC CTGTCTATTG CTCCATCTTG
781      GCTGCTGTGG TTGTGGGCCT TGTGGCCTAT ATTGCTTTCA AGAGATGGAA CAGCTGCAAG
841      CAAAATAAAC AAGGAGCCAA CAGCCGGCCG GTGAACCAGA CACCCCCACC AGAGGGAGAG
901      AAACTGCACA GCGACAGCGG CATCTCTGTG GACAGCCAGA GCCTGCACGA CCAGCAGACC
961      CACACACAGA CTGCCTCAGG CCAAGCCCTC AAGGGTGATG GCAACCTCTA CAGTAGCCTG
1021     CCCCTGACCA AGCGTGAGGA GGTCGAGAAG CTGCTCAATG GTGACACCTG GCGACATCTG
1081     GCAGGCGAGC TGGGCTACCA GCCGGAGCAT ATAGACTCCT TTACCCACGA GGCCTGCCCA
1141     GTCCGAGCCC TGCTGGCCAG CTGGGGTGCC CAGGACAGCG CGACGCTCGA TGCCCTTTTA
1201     GCCGCCCTGC GACGCATCCA GAGAGCTGAC ATTGTGGAGA GCCTGTGCAG CGAGTCCACT
1261     GCCACGTCCC CTGTGTGA

 

Quality Control:cDNA sequencing results is available upon request.