Mouse NGFR / P75 Protein (Cytokine)

Nerve growth factor receptor (TNFR superfamily, member 16) Protein Datasheet

NGFR / P75 Protein Product Information

• Synonym: RP23-67E18.6, LNGFR, Tnfrsf16, p75, p75NGFR, p75NTR
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse NGFR (Q9Z0W1) (Met 1-Asn 243) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

NGFR / P75 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 22
• Molecular Mass: The secreted recombinant mouse NGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a calculated molecular mass of 50.6 kDa. The apparent molecular mass of rmNGFR/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NGFR / P75 protein

NGFR / P75 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NGFR / P75 Protein Description

Nerve growth factor receptors(NGFRs or P75) include high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. High-affinity nerve growth factor receptor is also known as TrkA. Nerve growth factor binds with TrkA after being released from target cells, the NGF / TrkA complex is subsequently trafficked back to the cell body. NGFLow-affinity nerve growth factor receptor also named p75 neurotrophin receptor. All the four kinds of neurotrophins, including Nerve growth factor, Brain derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75NTR. It has been demonstrated that NGF acts through its low-affinity receptor p75NTR. Studies have proved that NGFR acts as a molecular signal swith that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

References

1. Chen LW. et al., 2008, CNS Neurol Disord Drug Targets. 7 (6): 512-23.
2. Deponti D. et al., 2009, Mol Biol Cell. 20 (16): 3620-7.
3. Ken-ichiro K. et al., 2004, J Biol Chem. 279 (3): 1703-12.