Canine NGF Gene cDNA Clone / ORF Clone

NGF cDNA Clone Datasheet

NGF cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001194950.1
• cDNA Size: 726
• Gene Synonym: NGFB; NGF
• Source: Canine

NGF cDNA Clone Description

• cDNA Description: ORF Clone of Canis lupus familiaris nerve growth factor (beta polypeptide) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NGF cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NGF cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NGF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCATGT TGTTCTACAC TCTGATCACA GCTCTTCTGA TCGGCATCCG GGCAGAACCG
61       CATCCAGAGA GCCATGTCCC AGCAGGACAC GCCATCCCCC ACGCCCACTG GACTAAGCTT
121      CAGCATTCCC TTGACACAGC CCTCCGCAGA GCCCGCAGCG CCCCGGCCGG GGCAATAGCT
181      GCCAGGGTGA CAGGGCAGAC CCGCAACATC ACTGTGGATC CCAAACTCTT TAAAAAGCGG
241      CGACTGCGTT CGCCCCGCGT GCTGTTCAGC ACGCACCCCC CACCTGTGGC TGCGGACGCT
301      CAGGACCTGG ACCTGGAGGC CGGCAGCACC GCCTCCGTCA ACAGGACTCA CAGGAGCAAG
361      CGGTCTTCGT CCCACCCTGT CTTCCACCGG GGGGAGTTCT CGGTGTGCGA CAGCGTCAGC
421      GTGTGGGTGG GCGACAAGAC CACAGCCACC GACATCAAGG GCAAGGAGGT GATGGTGCTG
481      GGAGAGGTGA ACATTAACAA CAGTGTGTTC AAACAGTACT TCTTTGAGAC CAAGTGCCGG
541      GACCCCACCC CCGTGGACAG CGGGTGCAGG GGCATCGACT CCAAGCACTG GAACTCCTAC
601      TGCACCACCA CCCACACCTT CGTCAAGGCG CTGACCATGG ACGGCAAGCA GGCTGCCTGG
661      CGGTTCATCC GGATCGACAC GGCCTGCGTG TGCGTGCTCA GCAGGAAGGC CGGGAGACGA
721      GCCTGA

 

Quality Control:cDNA sequencing results is available upon request.

NGF Gene Description

As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α , PDGF and TGF-β .Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

References

1. Ullrich A. et al., 1983, Nature. 303: 821-5.
2. Brain A. et al., 1999, J Neurosci. 19: 8207-18.
3. Einarsdottir E. et al., 2004, Hum Mol Genet. 13: 799-805.
4. Pedraza C E. et al., 2005, Am J Pathol. 166: 533-43.