|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus nerve growth factor (beta polypeptide) DNA.|
|KpnI + XhoI|
|His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat NGF Gene cDNA Clone (full-length ORF Clone)||RG80426-G|
|Rat NGF Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80426-G-F|
|Rat NGF Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80426-G-M|
|Rat NGF Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80426-G-N|
|Rat NGF Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80426-G-Y|
|Product name||Product name|
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.