β-NGF / Beta-NGF Protein (Native)

Nerve Growth Factor Protein ( β-NGF / Beta-NGF ) Datasheet

β-NGF / Beta-NGF Protein Product Information

• Synonym: NGF , Beta-NGF , HSAN5 , MGC161426 , MGC161428 , NGFB
• Protein Construction: A DNA sequence encoding the mature form of human β-NGF ( NP_002497.2 ) ( Ser 122- Arg 239 ) was expressed
• Source: Human
• Expression Host: CHO Cells

β-NGF / Beta-NGF Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: 1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 0.2-2 ng/ml.
  2. Measured in a cell survival assay using PC-12 rat pheochromocytoma cells. The ED₅₀ for this effect is typically 1.07 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 122
• Molecular Mass: The mature recombinant human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution
• Formulation: Lyophilized from sterile 20mM NaAc, 150mM NaCl , pH 5.5
• SDS-PAGE: NGF protein

β-NGF / Beta-NGF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

β-NGF / Beta-NGF Protein Description

As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α , PDGF and TGF-β .Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

References

1. Ullrich A. et al., 1983, Nature. 303: 821-5.
2. Brain A. et al., 1999, J Neurosci. 19: 8207-18.
3. Einarsdottir E. et al., 2004, Hum Mol Genet. 13: 799-805.
4. Pedraza C E. et al., 2005, Am J Pathol. 166: 533-43.