Mouse β-NGF / Beta-NGF Protein (Native)

Mouse Beta-Nerve Growth Factor ( Beta-NGF / NGF ) Protein

Beta-NGF / NGF Protein Product Information

• Synonym: Ngf , Ngfb
• Protein Construction: A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122 - Gly 241) was expressed, with an initial Met at the C-terminus.
• Source: Mouse
• Expression Host: CHO Cells

Beta-NGF / NGF Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Bio-activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
  The ED₅₀ for this effect is 0.2-2 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse NGF consists of 121 amino acids and has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
• SDS-PAGE: NGF protein

Beta-NGF / NGF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beta-NGF / NGF Protein Description

Mouse Beta-nerve growth factor, also known as Beta-NGF, and NGF, is a secreted protein which belongs to the NGF-beta family. As the first one of a series of neurotrophic factors, NGF is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α, PDGF and TGF-β. Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

References

1. Ullrich A. et al., 1983, Nature. 303: 821-5.
2. Brain A. et al., 1999, J. Neurosci. 19: 8207-18.
3. Einarsdottir E. et al., 2004, Hum Mol Genet. 13: 799-805.
4. Pedraza CE. et al., 2005, Am J Pathol. 166: 533-43.