Anti-NGF Antibody

Cat: 11050-R711
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All NGF Reagents
Anti-NGF Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-NGF Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human NGF
Has cross-reactivity with Mouse β-NGF (Catalog # 50385-MNAC) in ELISA assay
Immunogen
Recombinant Human NGF / NGFB Protein (Catalog#11050-HNAC)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human NGF / NGFB (rh NGF / NGFB; Catalog#11050-HNAC; NP_002497.2; Ser122-Arg239) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #R711
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-NGF Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Notes
Block In a functional ELISA which immobilized recombinant Human β-NGF (Catalog # 11050-HNAC) at 2μg/mL (100 µL/well) in the plate, the Rabbit anti-Human β-NGF Monoclonal Antibody (Catalog # 11050-R711) can block the binding of 5 μg/mL of biotinylated Human NTRK1/Fc Chimera Protein (Catalog # 11073-H03H) to human β-NGF, the EC50 is 3.84 μg/mL.
Neutralization The neutralization activity of antibody is Measured by its ability to neutralize β­NGF­induced proliferation in the TF­1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 0.048­0.19 µg/mL in the presence of 10 ng/mL Recombinant Human β­NGF.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-NGF Antibody (Rabbit Monoclonal antibody) Images
Human NGF/NGFB/beta-NGF Blocking/Neutralization (B/N) 5591
Cell Proliferation Induced by β­NGF was Neutralized by Human β­NGF Antibody. Recombinant Human β­NGF (Catalog 11050-HNAC) stimulates proliferation in the TF­1 human erythroleukemic cell line. Proliferation elicited by Recombinant Human β­NGF (10 ng/mL) is neutralized by increasing concentrations of Rabbit Anti­Human β­NGF Monoclonal Antibody (Catalog 11050-R711). The IC50 is typically 0.048­0.19 µg/mL.
Anti-NGF Antibody Alternative Names
Anti-Beta-NGF Antibody;Anti-HSAN5 Antibody;Anti-NGFB Antibody
NGF Background Information

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Full Name
nerve growth factor (beta polypeptide)
References
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-NGF Antibody Rabbit Monoclonal ELISA 50385-R016
    Anti-NGF Antibody Rabbit Polyclonal ELISA 50385-RP01
    Anti-NGF Antibody Rabbit Polyclonal ELISA 50385-T16
    Anti-NGF Antibody Rabbit Monoclonal ELISA 11050-R815
    Anti-NGF Antibody Rabbit Polyclonal WB,ELISA 11050-T48
    Anti-NGF Antibody Rabbit Polyclonal ELISA 11050-T16
    Anti-NGF Antibody Mouse Monoclonal ELISA 11050-MM06
    Anti-NGF Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 11050-RB64
    Anti-NGF Antibody Rabbit Monoclonal Neutralization 11050-RC6
    Anti-NGF Antibody Rabbit Monoclonal IHC-P 11050-RD03
    Anti-NGF Antibody Rabbit Monoclonal ELISA(Det) 11050-R422
    Anti-NGF Antibody Rabbit Polyclonal ELISA 11050-RP01
    Anti-NGF Antibody Mouse Monoclonal ELISA 11050-MM08
    Anti-NGF Antibody Rabbit Monoclonal ELISA 11050-R412
    Anti-NGF Antibody Rabbit Polyclonal ELISA 11050-RP02
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