Human NEU1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13268-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NEU1 cDNA Clone Datasheet

NEU1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC000722
cDNA Size:	1248
Gene Synonym:	NEU; NANH; SIAL1; NEU1
Source:	Human

NEU1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens sialidase 1 (lysosomal sialidase) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

NEU1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NEU1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NEU1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACTGGGG AGCGACCCAG CACGGCGCTC CCGGACAGAC GCTGGGGGCC GCGGATTCTG
61       GGCTTCTGGG GAGGCTGTAG GGTTTGGGTG TTTGCCGCGA TCTTCCTGCT GCTGTCTCTG
121      GCAGCCTCCT GGTCCAAGGC TGAGAACGAC TTCGGTCTGG TGCAGCCGCT GGTGACCATG
181      GAGCAACTGC TGTGGGTGAG CGGGAGACAG ATCGGCTCAG TGGACACCTT CCGCATCCCG
241      CTCATCACAG CCACTCCGCG GGGCACTCTT CTCGCCTTTG CTGAGGCGAG GAAAATGTCC
301      TCATCCGATG AGGGGGCCAA GTTCATCGCC CTGCGGAGGT CCATGGACCA GGGCAGCACA
361      TGGTCTCCTA CAGCGTTCAT TGTCAATGAT GGGGATGTCC CCGATGGGCT GAACCTTGGG
421      GCAGTAGTGA GCGATGTTGA GACAGGAGTA GTATTTCTTT TCTACTCCCT TTGTGCTCAC
481      AAGGCCGGCT GCCAGGTGGC CTCTACCATG TTGGTATGGA GCAAGGATGA TGGTGTTTCC
541      TGGAGCACAC CCCGGAATCT CTCCCTGGAT ATTGGCACTG AAGTGTTTGC CCCTGGACCG
601      GGCTCTGGTA TTCAGAAACA GCGGGAGCCA CGGAAGGGCC GCCTCATCGT GTGTGGCCAT
661      GGGACGCTGG AGCGGGACGG AGTCTTCTGT CTCCTCAGCG ATGATCATGG TGCCTCCTGG
721      CGCTACGGAA GTGGGGTCAG CGGCATCCCC TACGGTCAGC CCAAGCAGGA AAATGATTTC
781      AATCCTGATG AATGCCAGCC CTATGAGCTC CCAGATGGCT CAGTCGTCAT CAATGCCCGA
841      AACCAGAACA ACTACCACTG CCACTGCCGA ATTGTCCTCC GCAGCTATGA TGCCTGTGAT
901      ACACTAAGGC CCCGTGATGT GACCTTCGAC CCTGAGCTCG TGGACCCTGT GGTAGCTGCA
961      GGAGCTGTAG TCACCAGCTC CGGCATTGTC TTCTTCTCCA ACCCAGCACA TCCAGAGTTC
1021     CGAGTGAACC TGACCCTGCG ATGGAGCTTC AGCAATGGTA CCTCATGGCG GAAAGAGACA
1081     GTCCAGCTAT GGCCAGGCCC CAGTGGCTAT TCATCCCTGG CAACCCTGGA GGGCAGCATG
1141     GATGGAGAGG AGCAGGCCCC CCAGCTCTAC GTCCTGTATG AGAAAGGCCG GAACCACTAC
1201     ACAGAGAGCA TCTCCGTGGC CAAAATCAGT GTCTATGGGA CACTCTGA

Quality Control:cDNA sequencing results is available upon request.