Mouse NETO1 Gene cDNA Clone / ORF Clone

NETO1 cDNA Clone Datasheet

NETO1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_144946.4
• cDNA Size: 1602
• Gene Synonym: Btcl1; AI851453; C130005O10Rik; Neto1
• Source: Mouse

NETO1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus neuropilin (NRP) and tolloid (TLL)-like 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NETO1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NETO1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NETO1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCTATG GACGCAGTTT GTTTCACATT ATAGCAAGTT TAATCATCCT CCATTCTTCT
61       GGAGCAACCA AGAAAGGAAC AGAAAAACAA ATCACCCCAG AAACACAGAA GTCAGTGCAG
121      TGTGGAACGT GGACAAAGCA TGCAGAGGGA GGTGTCTTTA CGTCTCCCAA TTATCCCAGC
181      AAATATCCCC CAGACCGAGA GTGTGTCTAC ATCATAGAAG CTGCCCCAAG GCAGTGCATT
241      GAACTTTACT TTGATGAAAA ATACTCAATC GAACCATCTT GGGAGTGCAA ATTTGATCAT
301      ATTGAAGTTC GAGATGGACC CTTTGGCTTT TCTCCAATAA TTGGAAGGTT CTGTGGACAA
361      CAGAATCCAC CTGTAATAAA ATCTAGTGGA AGATTTCTGT GGATTAAATT TTTTGCTGAT
421      GGCGAGCTGG AGTCTATGGG ATTTTCAGCT CGATATAATT TCACACCTGA TCCCGACTTT
481      AAGGACCTTG GAGTTTTGAA ACCATTGCCA GCGTGTGAGT TTGAGATGGG CGGCCCGGAA
541      GGAATTGTGG AGTCCATACA GATTCTGAAG GAAGGCAAAG CTTCTGCCAG TGAAGCCGTG
601      GATTGCAAAT GGTACATCCG AGCCCCACCA CGATCCAAGA TTTACTTACG TTTCTTGGAC
661      TATGAGATGC AGAATTCAAA TGAGTGCAAA AGAAACTTTG TGGCGGTCTA TGACGGAAGC
721      AGCTCCGTGG AGGATTTGAA GGCCAAGTTC TGCAGCACAG TGGCTAATGA CGTCATGCTG
781      CGCACAGGCC TTGGGGTGAT CCGCATGTGG GCAGACGAGG GTAGCCGTAA CAGCAGGTTT
841      CAGATGCTCT TCACATCCTT TCAAGAACCT CCCTGTGAAG GCAACACATT CTTCTGCCAC
901      AGTAACATGT GTATTAACAA CACGCTAGTC TGCAATGGGC TCCAGAACTG TGTATATCCC
961      TGGGATGAAA ATCACTGTAA AGAAAAGAGG AAAACCAGCC TGCTGGATCA GCTGACCAAC
1021     ACCAGTGGGA CGGTCATTGG CGTGACTTCC TGCATTGTGA TCATCCTTAT TATCGTTTCT
1081     GTCATTGTAC AGATCAAACA GCCTCGTAAA AAATACGTCC AAAGGAAGTC AGACTTTGAC
1141     CAGACCGTTT TCCAGGAGGT GTTTGAGCCT CCCCACTATG AGTTATGTAC GCTCAGAGGA
1201     ACTGGAGCAA CAGCCGACTT TGCAGACGTG GCGGAAGACT TTGAAAATTA CCATAAACTG
1261     AGGAGATCAT CTTCCAAATG CATCCATGAC CATCACTGTG GATCCCAACT GTCCAGCGCG
1321     AAAGGCAGTC GCAGTAACCT CAGCACAAGG GACGCTTCCA TTTTGGCAGA GATACCCACA
1381     CAGCCAGTCA AGCCCCTCAT CCCACCCGTG AACAGAAGGA ACATCCTGGT CATGAAACAC
1441     AACTACTCAC AAGATGCCGC CGATGCCTGT GACATCGACG AGATTGAGGA GGTGCCCACC
1501     ACAAGCCACA GGCTATCCAG ACACGAAAAA TCTGTCCAGC GGTTCTGCCT CATTGGGTCT
1561     CTAAGCAAAC ATGAATCTGA ATACAACACA ACTAGGGTCT AA

 

Quality Control:cDNA sequencing results is available upon request.