NETO1 / BTCL1 Antibody

Neuropilin and Tolloid-like Protein 1 ( NETO1 / BTCL1 ) Antibody

NETO1 / BTCL1 Antibody Product Information

• Immunogen: Recombinant human NETO1 protein ( Catalog#11562-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human NETO1 ( rh NETO1 ; Catalog#11562-H08H ; NP_620416.1 ; Met 1 - Thr 344 ). Total IgG was purified by Protein A affinity chromatography

NETO1 / BTCL1 Antibody Usage Guide

• Specificity: Human NETO1 / BTCL1
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human NETO1 in WB
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human NETO1. The detection limit for human NETO1 is 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NETO1 / BTCL1 Antibody Background

Neuropilin and tolloid-like protein 1, also known as brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, NETO1 and BTCL1, is a single-pass type I membrane protein which contains two CUB domains and contains one LDL-receptor class A domain. Isoform 1 and isoform 2 of NETO1 / BTCL1 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain. NETO1 / BTCL1 interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. It interacts with GRIN2A and GRIN2B via its CUB domains. NETO1 / BTCL1 is involved in the development and/or maintenance of neuronal circuitry. It is an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. NETO1 / BTCL1 regulates long-term NMDA receptor-dependent synaptic plasticity and cognition in the context of spatial learning and memory.

References

1. Stoehr H. et al., 2002,Gene 286:223-231.
2. Ng,D. et al., 2009, PLoS Biol. 7 (2):e41.
3. O'Donnell,L. et al., 2010, Hum Genet. 128 (2):155-64.