Human NELL2 Gene cDNA Clone / ORF Clone

Human NELL2 cDNA Clone Datasheet

NELL2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001145107.1
• cDNA Size: 2601
• Gene Synonym: NRP2; NELL2
• Source: Human

NELL2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens NEL-like 2 (chicken) DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 240 T/C not causing the amino acid variation.

NELL2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NELL2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NELL2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTTCC CTCCTTTGCT TAAAGGGCAG GCGAGCGCTA CTCGCTCCAG CCTTGCCTCC
61       TGCAGCTGGG TGGTCTTTTT TCTCTCCTGT CTTTCAAGAC ACGCGCCCGA AATCGAGGGA
121      GGGAGACGAT GGACTGAGCT GATCCGCACC ATGGAGTCTC GGGTCTTACT GAGAACATTC
181      TGTTTGATCT TCGGTCTCGG AGCAGTTTGG GGGCTTGGTG TGGACCCTTC CCTACAGATC
241      GACGTCTTAA CAGAGTTAGA ACTTGGGGAG TCCACGACCG GAGTGCGTCA GGTCCCGGGG
301      CTGCATAATG GGACGAAAGC CTTTCTCTTT CAAGATACTC CCAGAAGCAT AAAAGCATCC
361      ACTGCTACAG CTGAACAGTT TTTTCAGAAG CTGAGAAATA AACATGAATT TACTATTTTG
421      GTGACCCTAA AACAGACCCA CTTAAATTCA GGAGTTATTC TCTCAATTCA CCACTTGGAT
481      CACAGGTACC TGGAACTGGA AAGTAGTGGC CATCGGAATG AAGTCAGACT GCATTACCGC
541      TCAGGCAGTC ACCGCCCTCA CACAGAAGTG TTTCCTTACA TTTTGGCTGA TGACAAGTGG
601      CACAAGCTCT CCTTAGCCAT CAGTGCTTCC CATTTGATTT TACACATTGA CTGCAATAAA
661      ATTTATGAAA GGGTAGTAGA AAAGCCCTCC ACAGACTTGC CTCTAGGCAC AACATTTTGG
721      CTAGGACAGA GAAATAATGC GCATGGATAT TTTAAGGGTA TAATGCAAGA TGTCCAATTA
781      CTTGTCATGC CCCAGGGATT TATTGCTCAG TGCCCAGATC TTAATCGCAC CTGTCCAACT
841      TGCAATGACT TCCATGGACT TGTGCAGAAA ATCATGGAGC TACAGGATAT TTTAGCCAAA
901      ACATCAGCCA AGCTGTCTCG AGCTGAACAG CGAATGAATA GATTGGATCA GTGCTATTGT
961      GAAAGGACTT GCACCATGAA GGGAACCACC TACCGAGAAT TTGAGTCCTG GATAGACGGC
1021     TGTAAGAACT GCACATGCCT GAATGGAACC ATCCAGTGTG AAACTCTAAT CTGCCCAAAT
1081     CCTGACTGCC CACTTAAGTC GGCTCTTGCG TATGTGGATG GCAAATGCTG TAAGGAATGC
1141     AAATCGATAT GCCAATTTCA AGGACGAACC TACTTTGAAG GAGAAAGAAA TACAGTCTAT
1201     TCCTCTTCTG GAGTATGTGT TCTCTATGAG TGCAAGGACC AGACCATGAA ACTTGTTGAG
1261     AGTTCAGGCT GTCCAGCTTT GGATTGTCCA GAGTCTCATC AGATAACCTT GTCTCACAGC
1321     TGTTGCAAAG TTTGTAAAGG TTATGACTTT TGTTCTGAAA GGCATAACTG CATGGAGAAT
1381     TCCATCTGCA GAAATCTGAA TGACAGGGCT GTTTGTAGCT GTCGAGATGG TTTTAGGGCT
1441     CTTCGAGAGG ATAATGCCTA CTGTGAAGAC ATCGATGAGT GTGCTGAAGG GCGCCATTAC
1501     TGTCGTGAAA ATACAATGTG TGTCAACACC CCGGGTTCTT TTATGTGCAT CTGCAAAACT
1561     GGATACATCA GAATTGATGA TTATTCATGT ACAGAACATG ATGAGTGTAT CACAAATCAG
1621     CACAACTGTG ATGAAAATGC TTTATGCTTC AACACTGTTG GAGGACACAA CTGTGTTTGC
1681     AAGCCGGGCT ATACAGGGAA TGGAACGACA TGCAAAGCAT TTTGCAAAGA TGGCTGTAGG
1741     AATGGAGGAG CCTGTATTGC CGCTAATGTG TGTGCCTGCC CACAAGGCTT CACTGGACCC
1801     AGCTGTGAAA CGGACATTGA TGAATGCTCT GATGGTTTTG TTCAATGTGA CAGTCGTGCT
1861     AATTGCATTA ACCTGCCTGG ATGGTACCAC TGTGAGTGCA GAGATGGCTA CCATGACAAT
1921     GGGATGTTTT CACCAAGTGG AGAATCGTGT GAAGATATTG ATGAGTGTGG GACCGGGAGG
1981     CACAGCTGTG CCAATGATAC CATTTGCTTC AATTTGGATG GCGGATATGA TTGTCGATGT
2041     CCTCATGGAA AGAATTGCAC AGGGGACTGC ATCCATGATG GAAAAGTTAA GCACAATGGT
2101     CAGATTTGGG TGTTGGAAAA TGACAGGTGC TCTGTGTGCT CATGTCAGAA TGGATTCGTT
2161     ATGTGTCGAC GGATGGTCTG TGACTGTGAG AATCCCACAG TTGATCTTTT TTGCTGCCCT
2221     GAATGTGACC CAAGGCTTAG TAGTCAGTGC CTCCATCAAA ATGGGGAAAC TTTGTATAAC
2281     AGTGGTGACA CCTGGGTCCA GAATTGTCAA CAGTGCCGCT GCTTGCAAGG GGAAGTTGAT
2341     TGTTGGCCCC TGCCTTGCCC AGATGTGGAG TGTGAATTCA GCATTCTCCC AGAGAATGAG
2401     TGCTGCCCGC GCTGTGTCAC AGACCCTTGC CAGGCTGACA CCATCCGCAA TGACATCACC
2461     AAGACTTGCC TGGACGAAAT GAATGTGGTT CGCTTCACCG GGTCCTCTTG GATCAAACAT
2521     GGCACTGAGT GTACTCTCTG CCAGTGCAAG AATGGCCACA TCTGTTGCTC AGTGGATCCA
2581     CAGTGCCTTC AGGAACTGTG A

 

Quality Control:cDNA sequencing results is available upon request.