NELL2 / NRP2 Protein (His Tag)

NEL-like 2 (chicken) Protein Datasheet

NELL2 / NRP2 Protein Product Information

• Synonym: NRP2
• Protein Construction: A DNA sequence encoding the human NELL2 (Q99435) （Met 1-Leu 816）was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

NELL2 / NRP2 Protein QC Testing

• Purity: > 87 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 22
• Molecular Mass: The secreted recombinant human NELL2 consists of 805 amino acids and predicts a molecular mass of 90.4 kDa. It migrates as an approximately 110 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NELL2 / NRP2 protein

NELL2 / NRP2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NELL2 / NRP2 Protein Description

Protein kinase C-binding protein NELL2 is a neuron-specific thrombospondin-1 like extracellular protein that contains epidermal growth factor (EGF) – like repeats and several von Willebrand factor C domains. NELL2 acts as a homotrimer and plays a key role in neural cell growth and differentiation as well as in oncogenesis. NELL2 is highly expressed in the hippocampus and cerebral cortex. This extracellular protein increased survival of neurons from the hippocampus and cerebral cortex and did not protect neurons from oxygen-glucose deprivation- and beta-amyloid-induced neuronal death. NELL2-enhanced survival of hippocampal neurons was completely blocked by SP600125, an anthrapyrazolone inhibitor of JNK, while treatment of MEK (MAPK / ERK kinase) inhibitors per se enhanced survival of neurons similar to NELL2 treatment. All of these suggest that NELL2 promotes survival of neurons by modulating MAPk activities. It has been proved that NELL2 is highly regulated spatially and temporally, being only transiently expressed in discrete regions of the central (CNS) an peripheral nervous systems (PNS) and in a subset of mesoderm derived structures during their peak periods of development.

References

1. Kuroda S. et al., 1999, Biochem Biophys Res Commun. 265 (1): 79-86.
2. Oyasu M. et al., 2000, Brain Res Mol Brain Res. 76 (1): 151-60.
3. Nelson BR. et al., 2002, Mech Dev. 119 (1): 11-9.