Mouse NEK3 Gene cDNA Clone / ORF Clone

NEK3 cDNA Clone Datasheet

NEK3 cDNA Clone Product Information

• Gene Bank Ref.ID: EF043796
• cDNA Size: 1530
• Gene Synonym: Nek3
• Source: Mouse

NEK3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NEK3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NEK3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NEK3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACAACT ACACAGTCCT GAGAGTGATT GGCCAGGGCT CCTTCGGCAG GGCTCTTTTG
61       GTTCTGCAGG AAAGCAGCAA TCAGACATTT GCCATGAAGG AAATCAGACT GCTCAAGTCT
121      GATACACAGA CTTCTAGGAA AGAGGCTGTT CTGCTCGCCA AAATGAAGCA CCCCAATATT
181      GTTGCCTTTA AGGAGTCATT TGAAGCTGAA GGGTACCTGT ACATTGTGAT GGAGTATTGT
241      GACGGAGGGG ATCTGATGCA GAGGATTAAA CAGCAAAAAG GGAATTTATT CCCTGAAGAC
301      ACGATACTTA ATTGGTTTAT ACAAATTTGT CTTGGAGTAA ATCACATTCA TAAGAGACGT
361      GTTTTACACA GAGATATCAA GTCGAAAAAT GTCTTCCTCA CCCACAATGG AAAAGTGAAA
421      TTGGGAGATT TTGGCTCTGC CCGTCTTCTC TCCAGTCCCA TGGCGTTTGC ATGCACGTAC
481      GTGGGAACAC CTTATTATGT GCCTCCAGAA ATCTGGGAGA ACCTGCCTTA CAACAATAAA
541      AGTGACATCT GGTCCTTGGG ATGCATTCTG TATGAACTCT GCGCTCTGAA GCATCCATTC
601      CAGGCAAATA GTTGGAAAAA CCTGATCCTG AAAATATGCC AGGGGCCCAT CCACCCGCTG
661      CCGGCTCTGT ACTCCTGCAA GCTTCAGGGC CTGGTCAAGC AGATGCTGAA AAGGAACCCT
721      TCGCACCGCC CCTCCGCCAC AACCCTGCTC TGCAGGGGTA GCTTGGCTCC ACTGGTCCCG
781      AAGTGCTTGC CCCCTCAGAT CATCAGAGAA TATGGAGAAC AGATATTAGA TGAAATCAAA
841      ATATCCACGC CTAAGAATAT GAAAAAACAG GACTCCAACA GAGTCGGGAG AGCTCTGGGA
901      GAAGCAAACT CTGCCGCCAT GCAAGAGGAA GAACGTGGTA GAAAGTGTAG CCATACTGAA
961      TTGGAAAGCA CTGGTACAAC CCCAGCTGGG AATGCATTGG GAAGAGCAGC CAGAGGAAAT
1021     CCAGGTAACC GACAGGAGCA CGGGAGCCAT ACCAGTCCGG CAAGTCCTCA CAGGCCGCGG
1081     TGGGAGAGAC ACGGTCCCAG TTCCAATGTC GAAGCTTTGG AAAAAGCATC TATCCTTACC
1141     TCCAGTTTCA CGGCAGAGGA TGACAGAGGT GGGTCTGTGA TAAAGTATGA AGAAAATGCC
1201     CGAAGGCAGT GGGTCAGAGA GCCCCCTGAG GCCTTGCTGT CAATGCTGAA GGACGCTGAT
1261     CTCAGTCAGG CATTTCAGAC ATACACAATA TATAGACCAG GTGCAGAAGG ATTCCTGAAA
1321     GGTCCCCTTT CTGAAGACAC GGCATCGGAC AGCGTCGACG GTGATCTTGA TTCTGTCATG
1381     TTAGACCCAG AGAGATTTGA ACCTCGGCTG GATGAAGAAG ACACGGACTT TGAGGAAGAT
1441     AACGAAAACC CTGATTGGGT GTCAGAACTA AAGAAGCACG TAGGATACGG GGATGGACCT
1501     GGTGGACAAC TCCTCGGAGA ACGTGCCTGA

 

Quality Control:cDNA sequencing results is available upon request.