NEK3

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.

NEK3 Related Areas

Cancer>>Cell Cycle>> NEK3

NEK3 Alternative Names

HSPK 36, glycogen synthase A kinase; hydroxyalkyl-protein kinase, never in mitosis A-related kinase 3, nimA-related protein kinase 3, phosphorylase B kinase kinase, serine/threonine-protein kinase Nek3 [Homo sapiens]

NIMA-related expressed kinase 3, never in mitosis A-related kinase 3, nimA-related protein kinase 3, serine/threonine-protein kinase Nek3 [Mus musculus]

Summaries for NEK3

Entrez Gene summary for NEK3:

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

OMIM - description for NEK3:

In Aspergillus nidulans, lack of the protein-serine/threonine kinase nimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. Schultz and Nigg (1993) isolated partial cDNAs representing 41 distinct protein kinases, including NEK3 and 2 other nimA-related kinases. See NEK2 (604043). Schultz et al. (1994) isolated additional NEK3 cDNAs corresponding to most of the coding region.

Wikipedia summary for NEK3:

Serine/threonine-protein kinase Nek3 is an enzyme that in humans is encoded by the NEK3 gene. In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized

Human NEK3 Protein General Information

 

• Protein names: Recommended name: Serine/threonine-protein kinase Nek3
• Sequence length: 506 AA.
• Sequence similarities: Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent.
• Post-translational modification: Phosphorylation at Thr-479 regulates its catalytic activity
• Subunit structure: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain.
• Subcellular location: Cytoplasm By similarity. Cell projection › axon
• Tissue specificity: Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain
• Involvement in disease

General information above from UniProt

Function for NEK3 Protein

UniProtKB:

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2

Genatlas:

• NEK3 may play a role in mitotic regulation

Homology for human NEK3

• ortholog to Aspergillus nidulans NIMA
• homolog to rattus Nek3 (75.4pc)
• homolog to murine Nek3 (75.1pc)