Human NEK11 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12324-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NEK11 cDNA Clone Datasheet

NEK11 cDNA Clone Product Information

Gene Bank Ref.ID:	BC028587
cDNA Size:	1449
Gene Synonym:	NEK11
Source:	Human

NEK11 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens NIMA (never in mitosis gene a)-related kinase 11 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

NEK11 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NEK11 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NEK11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGAAAT TCCAAGAGGC AGCTAAGTGT GTGAGTGGAT CAACAGCCAT TTCCACTTAT
61       CCAAAGACCT TGATTGCAAG AAGATACGTG CTTCAACAAA AACTTGGCAG TGGAAGTTTT
121      GGAACTGTCT ATCTGGTTTC AGACAAGAAA GCCAAACGAG GAGAGGAATT AAAGGTACTT
181      AAGGAAATAT CTGTTGGAGA ACTAAATCCA AATGAAACTG TACAGGCCAA TTTGGAAGCC
241      CAACTCCTCT CCAAGCTGGA CCACCCAGCC ATTGTCAAGT TCCATGCAAG TTTTGTGGAG
301      CAAGATAATT TCTGCATTAT CACGGAGTAC TGTGAGGGCC GAGATCTGGA CGATAAAATT
361      CAGGAATATA AACAAGCTGG AAAAATCTTT CCAGAAAATC AAATAATAGA ATGGTTTATC
421      CAGCTGCTGC TGGGAGTTGA CTACATGCAT GAGAGGAGGA TACTTCATCG AGACTTAAAG
481      TCAAAGAATG TATTTCTGAA AAATAATCTC CTTAAAATTG GAGATTTTGG AGTTTCTCGA
541      CTTCTAATGG GATCCTGTGA CCTGGCCACA ACTTTAACTG GAACTCCCCA TTATATGAGT
601      CCTGAGGCTC TGAAACACCA AGGCTATGAC ACAAAGTCGG ACATCTGGTC ACTGGCATGC
661      ATTTTGTATG AGATGTGCTG CATGAATCAT GCATTCGCTG GCTCCAATTT CTTATCCATT
721      GTTTTAAAAA TTGTTGAAGG TGACACACCT TCTCTCCCTG AGAGATATCC AAAAGAACTA
781      AATGCCATCA TGGAAAGCAT GTTGAACAAG AATCCTTCAT TAAGACCATC TGCTATCGAA
841      ATTTTAAAAA TCCCTTACCT TGATGAGCAG CTACAGAACC TAATGTGTAG ATATTCAGAA
901      ATGACTCTGG AAGACAAAAA TTTGGATTGT CAGAAGGAGG CTGCTCATAT AATTAATGCC
961      ATGCAAAAAA GGATCCACCT GCAGACTCTG AGGGCACTGT CAGAAGTACA GAAAATGACG
1021     CCAAGAGAAA GGATGCGGCT GAGGAAGCTC CAGGCGGCTG ATGAGAAAGC CAGGAAGCTG
1081     AAAAAGATTG TGGAAGAAAA ATATGAAGAA AATAGCAAAC GAATGCAAGA ATTGAGATCT
1141     CGGAACTTTC AGCAGCTGAG TGTTGATGTA CTCCATGAAA AAACACATTT AAAAGGAATG
1201     GAAGAAAAGG AGGAGCAACC TGAGGGAAGA CTTTCTTGTT CACCCCAGGA CGAGGATGAA
1261     GAGAGGTGGC AAGGCAGGGA AGAGGAATCT GATGAACCAA CTTTAGAGAA CCTGCCTGAG
1321     TCTCAGCCTA TTCCTTCCAT GGACCTCCAC GAACTTGAAT CAATTGTAGA GGATGCCACA
1381     TCTGACCTTG GATACCATGG AGACTGTAAT CTAATTTCAC TAGACGAATA CTGGAAAAAT
1441     GAAAAATAA

Quality Control:cDNA sequencing results is available upon request.