Human NEFM Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13209-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NEFM cDNA Clone Datasheet

NEFM cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001105541.1
cDNA Size:	1623
Gene Synonym:	NFM; NEF3; NF-M;NEFM
Source:	Human

NEFM cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens neurofilament, medium polypeptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 187 C/A resulting in the amino acid Pro substitution by Thr.

NEFM cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NEFM cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NEFM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCGTC ATTTGCGCGA ATACCAGGAC CTCCTCAACG TCAAGATGGC TCTGGATATA
61       GAAATCGCTG CGTACAGAAA ACTCCTGGAG GGTGAAGAGA CTAGATTTAG CACATTTGCA
121      GGAAGCATCA CTGGGCCACT GTATACACAC CGACCCCCAA TCACAATATC CAGTAAGATT
181      CAGAAAACCA AGGTGGAAGC TCCCAAGCTT AAGGTCCAAC ACAAATTTGT CGAGGAGATC
241      ATAGAGGAAA CCAAAGTGGA GGATGAGAAG TCAGAAATGG AAGAGGCCCT GACAGCCATT
301      ACAGAGGAAT TGGCCGTTTC CATGAAGGAA GAGAAGAAAG AAGCAGCAGA AGAAAAGGAA
361      GAGGAACCCG AAGCTGAAGA AGAAGAAGTA GCTGCCAAAA AGTCTCCAGT GAAAGCAACT
421      GCACCTGAAG TTAAAGAAGA GGAAGGGGAA AAGGAGGAAG AAGAAGGCCA GGAAGAAGAG
481      GAGGAAGAAG ATGAGGGAGC TAAGTCAGAC CAAGCCGAAG AGGGAGGATC CGAGAAGGAA
541      GGCTCTAGTG AAAAAGAGGA AGGTGAGCAG GAAGAAGGAG AAACAGAAGC TGAAGCTGAA
601      GGAGAGGAAG CCGAAGCTAA AGAGGAAAAG AAAGTGGAGG AAAAGAGTGA GGAAGTGGCT
661      ACCAAGGAGG AGCTGGTGGC AGATGCCAAG GTGGAAAAGC CAGAAAAAGC CAAGTCTCCT
721      GTGCCAAAAT CACCAGTGGA AGAGAAAGGC AAGTCTCCTG TGCCCAAGTC ACCAGTGGAA
781      GAGAAAGGCA AGTCTCCTGT GCCCAAGTCA CCAGTGGAAG AGAAAGGCAA GTCTCCTGTG
841      CCGAAATCAC CAGTGGAAGA GAAAGGCAAG TCTCCTGTGT CAAAATCACC AGTGGAAGAG
901      AAAGCCAAAT CTCCTGTGCC AAAATCACCA GTGGAAGAGG CAAAGTCAAA AGCAGAAGTG
961      GGGAAAGGTG AACAGAAAGA GGAAGAAGAA AAGGAAGTCA AGGAAGCTCC CAAGGAAGAG
1021     AAGGTAGAGA AAAAGGAAGA GAAACCAAAG GATGTGCCAG AGAAGAAGAA AGCTGAGTCC
1081     CCTGTAAAGG AGGAAGCTGT GGCAGAGGTG GTCACCATCA CCAAATCGGT AAAGGTGCAC
1141     TTGGAGAAAG AGACCAAAGA AGAGGGGAAG CCACTGCAGC AGGAGAAAGA GAAGGAGAAA
1201     GCGGGAGGAG AGGGAGGAAG TGAGGAGGAA GGGAGTGATA AAGGTGCCAA GGGATCCAGG
1261     AAGGAAGACA TAGCTGTCAA TGGGGAGGTA GAAGGAAAAG AGGAGGTAGA GCAGGAGACC
1321     AAGGAAAAAG GCAGTGGGAG GGAAGAGGAG AAAGGCGTTG TCACCAATGG CCTAGACTTG
1381     AGCCCAGCAG ATGAAAAGAA GGGGGGTGAT AAAAGTGAGG AGAAAGTGGT GGTGACCAAA
1441     ACGGTAGAAA AAATCACCAG TGAGGGGGGA GATGGTGCTA CCAAATACAT CACTAAATCT
1501     GTAACCGTCA CTCAAAAGGT TGAAGAGCAT GAAGAGACCT TTGAGGAGAA ACTAGTGTCT
1561     ACTAAAAAGG TAGAAAAAGT CACTTCACAC GCCATAGTAA AGGAAGTCAC CCAGAGTGAC
1621     TAA

Quality Control:cDNA sequencing results is available upon request.