Human NEFL Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13214-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NEFL cDNA Clone Datasheet

NEFL cDNA Clone Product Information

Gene Bank Ref.ID:	NM_006158.3
cDNA Size:	1632
Gene Synonym:	NFL; NF-L; NF68; CMT1F; CMT2E; FLJ53642;NEFL
Source:	Human

NEFL cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens neurofilament, light polypeptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

NEFL cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NEFL cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NEFL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG
61       ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCACG CTCAGCTTAC
121      TCCAGCTACT CGGCGCCGGT GTCTTCCTCG CTGTCCGTGC GCCGCAGCTA CTCCTCCAGC
181      TCTGGATCGT TGATGCCCAG TCTGGAGAAC CTCGACCTGA GCCAGGTAGC CGCCATCAGC
241      AACGACCTCA AGTCCATCCG CACGCAGGAG AAGGCGCAGC TCCAGGACCT CAATGACCGC
301      TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC
361      GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCATCCC GCTTCCGGGC GCTGTACGAG
421      CAGGAGATCC GCGACCTGCG CCTGGCGGCG GAAGATGCCA CCAACGAGAA GCAGGCGCTC
481      CAGGGCGAGC GCGAAGGGCT GGAGGAGACC CTGCGCAACC TGCAGGCGCG CTATGAAGAG
541      GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGCGCCGAC
601      GAGGCGGCGC TCGCTCGCGC CGAGCTCGAG AAGCGCATCG ACAGCTTGAT GGACGAAATC
661      TCTTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAAC TGCAGGCGCA GATCCAGTAC
721      GCGCAGATCT CCGTGGAGAT GGACGTGACC AAGCCCGACC TTTCCGCCGC GCTCAAGGAC
781      ATCCGCGCGC AGTACGAGAA GCTGGCCGCC AAGAACATGC AGAACGCTGA GGAATGGTTC
841      AAGAGCCGCT TCACCGTGCT GACCGAGAGC GCCGCCAAGA ACACCGACGC CGTGCGCGCC
901      GCCAAGGACG AGGTGTCCGA GAGCCGTCGT CTGCTCAAGG CCAAGACCCT GGAAATCGAA
961      GCATGCCGGG GCATGAATGA AGCGCTGGAG AAGCAGCTGC AGGAGCTGGA GGACAAGCAG
1021     AACGCCGACA TCAGCGCTAT GCAGGACACG ATCAACAAAT TAGAAAATGA ATTGAGGACC
1081     ACAAAGAGTG AAATGGCACG ATACCTAAAA GAATACCAAG ACCTCCTCAA CGTGAAGATG
1141     GCTTTGGATA TTGAGATTGC AGCTTACAGG AAACTCTTGG AAGGCGAGGA GACCCGACTC
1201     AGTTTCACCA GCGTGGGAAG CATAACCAGT GGCTACTCCC AGAGCTCCCA GGTCTTTGGC
1261     CGATCTGCCT ACGGCGGTTT ACAGACCAGC TCCTATCTGA TGTCCACCCG CTCCTTCCCG
1321     TCCTACTACA CCAGCCATGT CCAAGAGGAG CAGATCGAAG TGGAGGAAAC CATTGAGGCT
1381     GCCAAGGCTG AGGAAGCCAA GGATGAGCCC CCCTCTGAAG GAGAAGCCGA GGAGGAGGAG
1441     AAGGACAAGG AAGAGGCCGA GGAAGAGGAG GCAGCTGAAG AGGAAGAAGC TGCCAAGGAA
1501     GAGTCTGAAG AAGCAAAAGA AGAAGAAGAA GGAGGTGAAG GTGAAGAAGG AGAGGAAACC
1561     AAAGAAGCTG AAGAGGAGGA GAAGAAAGTT GAAGGTGCTG GGGAGGAACA AGCAGCTAAG
1621     AAGAAAGATT GA

Quality Control:cDNA sequencing results is available upon request.