Human NCSTN ORF Expression Clone, untagged

NCSTN cDNA Clone Datasheet

NCSTN cDNA Clone Product Information

• Gene Bank Ref.ID: NM_015331.2
• cDNA Size: 2130
• Gene Synonym: APH2; KIAA0253; RP11-517F10.1; NCSTN
• Source: Human

NCSTN cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens nicastrin DNA.
• Vector: pCMV/hygro
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

NCSTN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NCSTN cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NCSTN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTACGG CAGGGGGTGG CTCTGGGGCT GACCCGGGAA GTCGGGGTCT CCTTCGCCTT
61       CTGTCTTTCT GCGTCCTACT AGCAGGTTTG TGCAGGGGAA ACTCAGTGGA GAGGAAGATA
121      TATATCCCCT TAAATAAAAC AGCTCCCTGT GTTCGCCTGC TCAACGCCAC TCATCAGATT
181      GGCTGCCAGT CTTCAATTAG TGGAGACACA GGGGTTATCC ACGTAGTAGA GAAAGAGGAG
241      GACCTACAGT GGGTATTGAC TGATGGCCCC AACCCCCCTT ACATGGTTCT GCTGGAGAGC
301      AAGCATTTTA CCAGGGATTT AATGGAGAAG CTGAAAGGGA GAACCAGCCG AATTGCTGGT
361      CTTGCAGTGT CCTTGACCAA GCCCAGTCCT GCCTCAGGCT TCTCTCCTAG TGTACAGTGC
421      CCAAATGATG GGTTTGGTGT TTACTCCAAT TCCTATGGGC CAGAGTTTGC TCACTGCAGA
481      GAAATACAGT GGAATTCGCT GGGCAATGGT TTGGCTTATG AAGACTTTAG TTTCCCCATC
541      TTTCTTCTTG AAGATGAAAA TGAAACCAAA GTCATCAAGC AGTGCTATCA AGATCACAAC
601      CTGAGTCAGA ATGGCTCAGC ACCAACCTTC CCACTATGTG CCATGCAGCT CTTTTCACAC
661      ATGCATGCTG TCATCAGCAC TGCCACCTGC ATGCGGCGCA GCTCCATCCA AAGCACCTTC
721      AGCATCAACC CAGAAATCGT CTGTGACCCC CTGTCTGATT ACAATGTGTG GAGCATGCTA
781      AAGCCTATAA ATACAACTGG GACATTAAAG CCTGACGACA GGGTTGTGGT TGCTGCCACC
841      CGGCTGGATA GTCGTTCCTT TTTCTGGAAT GTGGCCCCAG GGGCTGAAAG CGCAGTGGCT
901      TCCTTTGTCA CCCAGCTGGC TGCTGCTGAA GCTTTGCAAA AGGCACCTGA TGTGACCACC
961      CTGCCCCGCA ATGTCATGTT TGTCTTCTTT CAAGGGGAAA CTTTTGACTA CATTGGCAGC
1021     TCGAGGATGG TCTACGATAT GGAGAAGGGC AAGTTTCCCG TGCAGTTAGA GAATGTTGAC
1081     TCATTTGTGG AGCTGGGACA GGTGGCCTTA AGAACTTCAT TAGAGCTTTG GATGCACACA
1141     GATCCTGTTT CTCAGAAAAA TGAGTCTGTA CGGAACCAGG TGGAGGATCT CCTGGCCACA
1201     TTGGAGAAGA GTGGTGCTGG TGTCCCTGCT GTCATCCTCA GGAGGCCAAA TCAGTCCCAG
1261     CCTCTCCCAC CATCTTCCCT GCAGCGATTT CTTCGAGCTC GAAACATCTC TGGCGTTGTT
1321     CTGGCTGACC ACTCTGGTGC CTTCCATAAC AAATATTACC AGAGTATTTA CGACACTGCT
1381     GAGAACATTA ATGTGAGCTA TCCCGAATGG CTGAGCCCTG AAGAGGACCT GAACTTTGTA
1441     ACAGACACTG CCAAGGCCCT GGCAGATGTG GCCACGGTGC TGGGACGTGC TCTGTATGAG
1501     CTTGCAGGAG GAACCAACTT CAGCGACACA GTTCAGGCTG ATCCCCAAAC GGTTACCCGC
1561     CTGCTCTATG GGTTCCTGAT TAAAGCCAAC AACTCATGGT TCCAGTCTAT CCTCAGGCAG
1621     GACCTAAGGT CCTACTTGGG TGACGGGCCT CTTCAACATT ACATCGCTGT CTCCAGCCCC
1681     ACCAACACCA CTTATGTTGT ACAGTATGCC TTGGCAAATT TGACTGGCAC AGTGGTCAAC
1741     CTCACCCGAG AGCAGTGCCA GGATCCAAGT AAAGTCCCAA GTGAAAACAA GGATCTGTAT
1801     GAGTACTCAT GGGTCCAGGG CCCTTTGCAT TCTAATGAGA CGGACCGACT CCCCCGGTGT
1861     GTGCGTTCTA CTGCACGATT AGCCAGGGCC TTGTCTCCTG CCTTTGAACT GAGTCAGTGG
1921     AGCTCTACTG AATACTCTAC ATGGACTGAG AGCCGCTGGA AAGATATCCG TGCCCGGATA
1981     TTTCTCATCG CCAGCAAAGA GCTTGAGTTG ATCACCCTGA CAGTGGGCTT CGGCATCCTC
2041     ATCTTCTCCC TCATCGTCAC CTACTGCATC AATGCCAAAG CTGATGTCCT TTTCATTGCT
2101     CCCCGGGAGC CAGGAGCTGT GTCATACTAA

 

Quality Control:cDNA sequencing results is available upon request.