Mouse NCSTN / Nicastrin Protein

Nicastrin Protein Datasheet

NCSTN / Nicastrin Protein Product Information

• Synonym: NCSTN
• Protein Construction: A DNA sequence encoding the mouse NCSTN (P57716) (Met1- Glu668) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

NCSTN / Nicastrin Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 28
• Molecular Mass: The recombinant mouse NCSTN/Fc is a disulfide-linked homodimer. The reduced monomer comprises 882 amino acids and has a predicted molecular mass of 98.5 kDa. The apparent molecular mass of the protein is approximately 117 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NCSTN / Nicastrin protein

NCSTN / Nicastrin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NCSTN / Nicastrin Protein Description

Nicastrin, also known as NCSTN, is a single-pass type I  membrane protein which belongs to the nicastrin family. Nicastrin was named after the Italian village Nicastro, reflecting the fact that Alzheimer's disease was described in 1963 after studying descendants of an extended family originating in the village of Nicastro that had Familial Alzheimer's Disease (FAD). Nicastrin is a protein that is part of the gamma secretase protein complex, which is one of the proteases involved in processing amyloid precursor protein (APP) to the short Alzheimer's disease-associated peptide amyloid beta. Nicastrin may represent a stabilizing cofactor required for the assembly of the gamma-secretase complex. The other proteins in the complex are presenilin, which is the catalytically active component of the complex, APH-1, and PEN-2. Nicastrin itself is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the complex, all of which undergo significant post-translational modification before becoming active in the cell. Nicastrin has also been identified as a regulator of neprilysin, an enzyme involved in the degradation of amyloid beta fragment.

References

1. Feldman RG. et al., 1963, Neurology. 13: 811-24.
2. Satoh J. et al., 2001, Neuropathology. 21: 115-22.
3. Zhang YW. et al., 2005, J Biol Chem. 280 (17): 17020-6.
4. Kaether C. et al., 2006, Neurodegener Dis. 3 (4-5): 275-83.
5. Chi A. et al., 2006, J. Proteome Res. 5: 3135-44.