|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus natural cytotoxicity triggering receptor 1 DNA.|
|Ly94, Nkp46, KILR-1, Nk-p46, Ncr1|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 255 T/C not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat NCR1 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80378-G-F|
|Rat NCR1 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80378-G-H|
|Rat NCR1 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80378-G-M|
|Rat NCR1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80378-G-N|
|Rat NCR1 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80378-G-Y|
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NCR1, also known as NK-p46 and CD335, is a natural cytotoxicity receptor(NCR). NCRs are type I transmembrane proteins with 1-2 extracellular immunoglobulin domains, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. All are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium.