Rat NCR1 Gene cDNA Clone / ORF Clone

NCR1 cDNA Clone Datasheet

NCR1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_057199.1
• cDNA Size: 978
• Gene Synonym: Ly94; Nkp46; KILR-1; Nk-p46; Ncr1
• Source: Rat

NCR1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus natural cytotoxicity triggering receptor 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 255 T/C not causing the amino acid variation.

NCR1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NCR1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NCR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCCAA CACTCACTGC CCTACTCTGC CTTGGTCTAT GTCTGAGCCA AAGGATCAAC
61       ACTGAAAAAC AGACTCTCCC AAAACCCATC ATCTGGGCCA AACCTAGTAT CATGGTCACA
121      AAAGGAAACT CGGTGAACAT CTGGTGTCAG GGGGCTCAGA GTGCTTCTGA GTATCAACTG
181      TACTTTGAAG GAAGCTTTTT TGCCTTGGAG AGACCAAAGT CATCGAGATC GATGAATAAA
241      GTCAAGTTCT TCATCTCACA AATGACCTCA CATACTGCAG GGATCTACAC CTGCTTCTAT
301      CAGAGTGGAG AGCTCTGGTC AGAGTCAAGC AACCCCCTGA AACTGGTGGT AACTGGTCTG
361      TATGACACAC CCACCCTCTG GGTTCACCCA GGGCCTGAGG TAACCTTGGG GGAGAATGTT
421      ACCTTCTCTT GCCATCTGAA GACTGCAACA AGCAAATTCT TTCTGCTCAA GGAGAGAGAA
481      TCCAACCATA TCCAGCATAA ATATGGGAAC ATCCAAGCAG AGTTCCCCAT GGGCCCTGTG
541      ACTAGAGCTC ACAGAGGGAC ATACAGATGT TTTGGCTCTT ACAACGACTA TGCATGGTCT
601      TTTCCCAGTG AGCCTGTGAC ACTACTCATC ACAGGAGAGG TTGAGAACAC CAGCCTTGCA
661      CCCACAGACC CTGTTTCTTC TCTTGATTAT TGGGAGTTTG ACCTTTCAAC CAAAGAATCA
721      GGATTGCAGA AGGACTCTGC CTTCTGGGAT CACACAGCCC AGAATCTCAT TCGGATTGGC
781      CTGGCATGCA TAATCGTGAT GGCTCTAGTA TGGCTTTTGG CTGAAGACTG GCTCAGCAGG
841      AGGAAGGATC ATGAGAAGCT CAATAGATTA ACAAGTTGGG AATGCAGGGG AAGACGGAGA
901      ATGCATCGTT ACCATGAAGA GGAGCAAAGA GATGCAATAT CTATGAGGGA ACTGAAGGCA
961      ACTCCTGGGG ATATGTGA

 

Quality Control:cDNA sequencing results is available upon request.