Rat NCR1 Protein

Natural cytotoxicity triggering receptor 1 Protein Datasheet

NCR1 Protein Product Information

• Synonym: NCR1, Ar1, Kilr1, Ly94
• Protein Construction: A DNA sequence encoding the rat NCR1 (Q9Z0H5) (Met1-Asn255) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

NCR1 Protein QC Testing

• Purity: (73.6+23)%as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 17
• Molecular Mass: The recombinant rat NCR1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 480 amino acids and has a predicted molecular mass of 54.1 kDa. The apparent molecular mass of the protein is approximately 65 and 36 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NCR1 protein

NCR1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NCR1 Protein Description

NCR1, also known as NK-p46 and CD335, is called natural cytotoxicity receptors (NCR) together with NCR3 and NCR2. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. It has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium. NKp46 is a surface receptor involved in NK-cell cell death by apoptosis. NKp46 and its coreceptor CD59 together activate cytotoxicity of human NK-cells, their engagement results in tyrosine phosphorylation of CD3-zeta chains associated with NKp46.

References

1. Carbone E. et al., 2005, Blood. 105 (1): 251-8.
2. Sivori S. et al., 1997, J Exp Med. 186 (7): 1129-36.
3. Biassoni R. et al., 2004, J Cell Mol Med. 7 (4): 376-87.