Rat NCAM1 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80399-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NCAM1 cDNA Clone Datasheet

NCAM1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_031521.1
cDNA Size:	2577
Gene Synonym:	Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1; MGC124601; Ncam1
Source:	Rat

NCAM1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus neural cell adhesion molecule 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1042A/G resulting in the amino acid asn substitution by asp.

NCAM1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NCAM1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NCAM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCGAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCCCTG
61       CAGGTAGATA TTGTTCCCAG CCAAGGAGAA ATCAGCGTTG GAGAGTCCAA ATTCTTCCTG
121      TGTCAAGTGG CAGGAGATGC CAAAGATAAG GACATCTCCT GGTTCTCCCC CAACGGGGAG
181      AAACTGAGCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ACGATGATGA CTCCTCTACC
241      CTCACCATCT ACAACGCCAA CATTGATGAT GCCGGCATTT ACAAGTGCGT GGTCACCGCT
301      GAAGACGGCA CCCAGTCCGA GGCCACTGTC AATGTGAAGA TCTTCCAGAA GCTCATGTTC
361      AAGAATGCGC CAACCCCACA GGAGTTTAAG GAAGGGGAAG ATGCTGTGAT TGTCTGTGAT
421      GTGGTCAGCT CTCTGCCCCC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
481      AAAAAAGATG TCCGGTTCAT AGTCCTATCC AACAACTACC TGCAGATCCG AGGCATCAAG
541      AAAACAGATG AGGGCACTTA CCGCTGTGAA GGCAGGATCC TGGCCCGGGG GGAGATCAAC
601      TTCAAGGACA TTCAGGTCAT TGTGAATGTA CCACCCACTG TCCAGGCCAG ACAGAGCATC
661      GTGAATGCCA CTGCCAACCT GGGCCAGTCT GTCACCCTGG TGTGTGATGC CGATGGCTTC
721      CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACCCA TAGAGAATGA GGAGGAAGAT
781      GACGAGAAGC ACATCTTCAG TGACGACAGT TCGGAGCTGA CCATCAGGAA TGTGGACAAA
841      AATGACGAAG CCGAATACGT CTGCATCGCT GAGAACAAGG CTGGCGAGCA GGATGCCTCC
901      ATCCACCTCA AGGTCTTCGC AAAGCCCAAA ATCACCTATG TAGAGAATCA GACAGCCATG
961      GAACTAGAGG AGCAAGTCAC TCTGACATGT GAAGCCTCCG GAGACCCCAT TCCTTCCATC
1021     ACCTGGAGAA CGTCCACCCG AGACATCAGC AGTGAAGAAA AGGCATCGTG GACTCGACCA
1081     GAGAAGCAAG AGACTCTAGA TGGGCACATG GTGGTACGCA GCCATGCTCG TGTGTCCTCC
1141     CTGACCCTGA AGAGCATCCA GTACACAGAT GCTGGAGAAT ACATCTGCAC TGCCAGCAAC
1201     ACCATCGGCC AGGACTCCCA GTCCATGTAC CTTGAAGTTC AATATGCTCC CAAGCTCCAG
1261     GGCCCTGTAG CTGTGTACAC TTGGGAAGGG AACCAGGTGA ACATCACCTG TGAGGTCTTT
1321     GCCTACCCAA GTGCCACAAT CTCCTGGTTC CGAGATGGCC AGTTGCTGCC AAGCTCCAAC
1381     TACAGCAATA TCAAGATCTA CAACACCCCA TCTGCGAGCT ATCTGGAGGT AACCCCTGAT
1441     TCCGAAAATG ACTTTGGAAA CTACAACTGC ACAGCGGTGA ACCGTATTGG ACAGGAGTCC
1501     TTGGAATTCA TCCTGGTTCA AGCAGATACA CCATCTTCCC CATCCATCGA CCGGGTGGAA
1561     CCATACTCCA GCACAGCACA GGTACAATTT GATGAGCCAG AAGCCACAGG TGGAGTTCCC
1621     ATCCTCAAAT ACAAGGCTGA GTGGAAGTCG CTGGGTGAAG AAGCATGGCA TTCCAAGTGG
1681     TATGATGCCA AAGAAGCCAA CATGGAAGGG ATTGTCACCA TCATGGGCCT GAAGCCTGAG
1741     ACAAGGTACG CGGTACGACT GGCGGCCCTC AACGGCAAGG GGCTGGGCGA GATCAGTGCA
1801     GCCACTGAGT TCAAGACACA GCCAGTCCGG GAACCCAGCG CACCCAAGCT GGAAGGGCAG
1861     ATGGGAGAGG ACGGGAACTC CATCAAGGTG AACCTGATCA AGCAGGATGA CGGCGGCTCC
1921     CCCATCAGAC ACTATCTGGT CAAGTACAGA GCGCTCGCCT CCGAGTGGAA ACCAGAGATC
1981     AGGCTCCCGT CCGGCAGTGA CCACGTCATG CTCAAGTCCC TAGACTGGAA CGCCGAGTAC
2041     GAAGTATATG TGGTAGCTGA GAACCAGCAA GGAAAATCCA AGGCAGCTCA CTTCGTGTTC
2101     AGGACTTCAG CCCAGCCCAC GGCCATCCCA GCCAATGGCA GCCCCACTGC AGGCCTGAGC
2161     ACAGGCGCCA TTGTGGGCAT CCTCATTGTC ATTTTCGTCC TACTCCTGGT GGTCATGGAC
2221     ATCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGCTCATGT GCATCGCTGT TAACCTGTGC
2281     GGCAAAGCGG GGCCCGGAGC CAAGGGCAAA GACATGGAGG AGGGCAAGGC TGCTTTCTCG
2341     AAAGATGAGT CTAAAGAACC CATTGTAGAG GTCCGAACGG AGGAGGAACG GACTCCAAAC
2401     CATGACGGAG GGAAGCACAC AGAGCCCAAC GAGACCACAC CACTGACAGA GCCCGAGAAG
2461     GGTCCTGTAG AAACAAAGTC CGAGCCCCAG GAGTCAGAAG CAAAGCCAGC GCCAACTGAA
2521     GTCAAGACGG TCCCCAATGA AGCCACACAA ACGAAAGAGA ATGAGAGCAA AGCATGA

Quality Control:cDNA sequencing results is available upon request.

Cytokine

TGF-beta