|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus neural cell adhesion molecule 1 DNA.|
|Cd56, Ncam, N-CAM, NCAMC, NCAM-1, NCAM-C, N-CAM-1, MGC124601, Ncam1|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 1042 A/G resulting in the amino acid Asn substitution by Asp.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat NCAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80399-G-F|
|Rat NCAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80399-G-H|
|Rat NCAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80399-G-M|
|Rat NCAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80399-G-N|
|Rat NCAM1 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80399-G-Y|
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NCAM1, also known as CD56, is a neural adhesion protein (NCAM) which belongs to the immunoglobulin superfamily. NCAM is involved in neural development and in plasticity in the adult brain. UCHL1 is a novel interaction partner of both NCAM isoforms that regulates their ubiquitination and intracellular trafficking. NCAM1 is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. NCAM1 has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.