Mouse DAN/NBL1 Gene cDNA Clone / ORF Clone

NBL1 cDNA Clone Datasheet

NBL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008675.2
• cDNA Size: 537
• Gene Synonym: DAN; NO3; Dana; MGC123430; D4H1S1733E; Nbl1
• Source: Mouse

NBL1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus neuroblastoma, suppression of tumorigenicity 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 550 T/C resulting in the amino acid CYS substitution by arg.

NBL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NBL1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NBL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTTGGG TCCTGGTGGG GGCCGTCCTC CCTGTCATGT TGCTGGCTGC CCCGCCACCT
61       ATCAACAAGC TGGCCCTGTT TCCGGACAAG AGTGCCTGGT GTGAAGCCAA GAACATCACG
121      CAGATTGTGG GCCACAGCGG CTGTGAGGCC AAGTCTATCC AGAACAGGGC GTGCCTAGGA
181      CAATGCTTCA GTTACAGCGT CCCCAACACC TTCCCGCAGT CCACAGAGTC CCTGGTGCAC
241      TGTGACTCCT GCATGCCGGC CCAGTCCATG TGGGAGATTG TGACCTTGGA GTGCCCTGGC
301      CACGAGGAGG TGCCCAGGGT GGACAAGCTG GTAGAGAAGA TTGTACACTG CAGCTGCCAG
361      GCTTGCGGCA AGGAGCCCAG TCACGAGGGC CTGAACGTCT ATGTGCAGGG TGAAGACAGC
421      CCGGGCTCCC AGCCTGGACC ACACTCCCAC GCCCATCCCC ACCCTGGGGG CCAGACCCCT
481      GAGCCCGAAG AGCCTCCTGG AGCCCCTCAG GTTGAGGAAG AGGGGGCTGA GGACTGA

 

Quality Control:cDNA sequencing results is available upon request.