NBL1 / DAN Protein & Antibody (DAND1)

Neuroblastoma, suppression of tumorigenicity 1

NBL1 / DAN Products

NBL1 / DAN Protein, Recombinant

Molecule	Species	Description	Cat. No
NBL1/DAN	Human	NBL1/DAN/Fc Protein, Recombinant	10169-H02H
NBL1/DAN	Human	NBL1/DAN Protein, Recombinant	10169-H08H
NBL1/DAN	Mouse	NBL1 / DAN Protein, Recombinant	50976-M08H

  10169-H02H:  The ED50 for this effect is typically 0.75 µg/mL in the presence of 100 ng/mL of recombinant human BMP4. Measured by its ability to inhibit BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells.

  10169-H08H:  The ED50 for this effect is typically >10 µg/mL in the presence of 50 ng/mL of recombinant human BMP4. Measured by its ability to inhibit BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells.

NBL1 / DAN Antibody

Molecule	Application	Description	Cat. No
Human NBL1/DAN	WB, ELISA	Mouse Monoclonal Antibody	10169-MM05
Human NBL1/DAN	WB, ELISA	Rabbit Polyclonal Antibody	10169-RP01
Human NBL1/DAN	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10169-RP02

NBL1 / DAN cDNA Clone

Molecule	Species	Description	Cat. No
NBL1/DAN	Human	Homo sapiens NBL1/DAN transcript variant 2 cDNA Clone(NM_005380.4)	HG10169-M
NBL1/DAN	Mouse	Mouse NBL1/DAN cDNA Clone / ORF Clone	MG50976-G

NBL1 / DAN Alternative Names

NBL1, DAND1, DAN, RP5-1056L3.4, D1S1733E, MGC8972, NB, NO3 [Homo sapiens]

Nbl1, Dana, DAN, RP23-65B16.3, D4H1S1733E, MGC123430, NO3 [Mus musculus]

NBL1 / DAN Related Areas

Cancer>>Growth Factor & Receptor>>TGF-beta Superfamily>>BMP Family>>NBL1/DAN

Immunology>>Cytokine & Receptor>>TGF-beta Superfamily>> TGF-beta Superfamily Modulators>>NBL1/DAN

NBL1 / DAN Related Products

Protein Products

Molecule	Species	Description
BMP-2	Human	BMP-2/Fc Protein, Recombinant
BMP-2	Human	BMP-2 Protein, Recombinant
BMP-5	Human	BMP-5/Fc Protein, Recombinant
ALK-3/BMPR1A	Human	ALK-3/BMPR1A/Fc Protein, Recombinant
ALK-3/BMPR1A	Human	ALK-3/BMPR1A/Fc Protein, Recombinant
BMPR2	Human	BMPR2/Fc Protein, Recombinant
BMPR2	Human	BMPR2 Protein, Recombinant
FLRG/FSTL3	Mouse	FLRG/FSTL3 Protein, Recombinant
Noggin	Human	Noggin/Fc Protein, Recombinant
Sclerostin/SOST	Human	Sclerostin/SOST Protein, Recombinant

Antibody Products

Molecule	Application	Description
Human Sclerostin/SOST	WB	Mouse Monoclonal Antibody
Human Sclerostin/SOST	ELISA	Rabbit Polyclonal Antibody

cDNA Clone Products

Molecule	Species	Description
BMP-2	Human	Homo sapiens BMP-2 cDNA Clone(NM_001200.2)
BMP-3b/GDF-10	Human	Homo sapiens BMP-3b/GDF-10 cDNA Clone(NM_004962.2)
BMP-4	Human	Homo sapiens BMP-4 cDNA Clone(NM_001202.3)
BMP-5	Human	Homo sapiens BMP-5 cDNA Clone(NM_021073.2)
BMP-7	Human	Homo sapiens BMP-7 cDNA Clone(NM_001719.2)
GDF-2/BMP-9	Human	Homo sapiens GDF-2/BMP-9 cDNA Clone(NM_016204.1)
ALK-3/BMPRIA	Human	Homo sapiens ALK-3/BMPR1A cDNA Clone(NM_004329.2)
ALK-6/BMPR1B	Human	Homo sapiens ALK-6/BMPR1B cDNA Clone(NM_001203.2)
BMPR2	Human	Homo sapiens BMPR2 cDNA Clone(NM_001204.6)
BAMBI/NMA	Human	Homo sapiens BAMBI/NMA cDNA Clone(NM_012342.2)
Cerberus 1	Human	Homo sapiens Cerberus 1 cDNA Clone(NM_005454.2)
Chordin-Like 1/CHRDL1	Human	Homo sapiens CHRDL1 cDNA Clone(NM_145234.2 )
CER2/DAND5	Human	Homo sapiens CER2/DAND5 cDNA Clone(NM_152654.2)
Follistatin	Human	Homo sapiens Follistatin cDNA Clone(NM_013409.1)
Noggin	Human	Homo sapiens Noggin cDNA Clone(NM_005450.4)
Tsukushin/TSK	Human	Homo sapiens Tsukushin/TSK cDNA Clone(NM_015516.3)

NBL1 / DAN Background

The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.

NBL1 / DAN Related Studies

1. Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.
2. Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.
3. Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.
4. Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.