Human NAPSA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13807-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NAPSA cDNA Clone Datasheet

NAPSA cDNA Clone Product Information

Gene Bank Ref.ID:	BC017842
cDNA Size:	1263
Gene Synonym:	KAP, Kdap, NAP1, NAPA, SNAPA,NAPSA
Source:	Human

NAPSA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens napsin A aspartic peptidase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

NAPSA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NAPSA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NAPSA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTCCAC CACCGCTGCT GCAACCCCTG CTGCTGCTGC TGCCTCTGCT GAATGTGGAG
61       CCTTCCGGGG CCACACTGAT CCGCATCCCT CTTCATCGAG TCCAACCTGG ACGCAGGACC
121      CTGAACCTAC TGAGGGGATG GAGAGAACCA GCAGAGCTCC CCAAGTTGGG GGCCCCATCC
181      CCTGGGGACA AGCCCATCTT CGTACCTCTC TCGAACTACA GGGATGTGCA GTATTTTGGG
241      GAAATTGGGC TGGGAACGCC TCCACAAAAC TTCACTGTTG CCTTTGACAC TGGCTCCTCC
301      AATCTCTGGG TCCCGTCCAG GAGATGCCAC TTCTTCAGTG TGCCCTGCTG GTTACACCAC
361      CGATTTGATC CCAAAGCCTC TAGCTCCTTC CAGGCCAATG GGACCAAGTT TGCCATTCAA
421      TATGGAACTG GGCGGGTAGA TGGAATCCTG AGCGAGGACA AGCTGACTAT TGGTGGAATC
481      AAGGGTGCAT CAGTGATTTT CGGGGAGGCT CTCTGGGAGC CCAGCCTGGT CTTCGCTTTT
541      GCCCATTTTG ATGGGATATT GGGCCTCGGT TTTCCCATTC TGTCTGTGGA AGGAGTTCGG
601      CCCCCGATGG ATGTACTGGT GGAGCAGGGG CTATTGGATA AGCCTGTCTT CTCCTTTTAC
661      CTCAACAGGG ACCCTGAAGA GCCTGATGGA GGAGAGCTGG TCCTGGGGGG CTCGGACCCG
721      GCACACTACA TCCCACCCCT CACCTTCGTG CCAGTCACGG TCCCCGCCTA CTGGCAGATC
781      CACATGGAGC GTGTGAAGGT GGGCCCAGGG CTGACTCTCT GTGCCAAGGG CTGTGCTGCC
841      ATCCTGGATA CGGGCACGTC CCTCATCACA GGACCCACTG AGGAGATCCG GGCCCTGCAT
901      GCAGCCATTG GGGGAATCCC CTTGCTGGCT GGGGAGTACA TCATCCTGTG CTCGGAAATC
961      CCAAAGCTCC CCGCAGTCTC CTTCCTTCTT GGGGGGGTCT GGTTTAACCT CACGGCCCAT
1021     GATTACGTCA TCCAGACTAC TCGAAATGGC GTCCGCCTCT GCTTGTCCGG TTTCCAGGCC
1081     CTGGATGTCC CTCCGCCTGC AGGGCCCTTC TGGATCCTCG GTGACGTCTT CTTGGGGACG
1141     TATGTGGCCG TCTTCGACCG CGGGGACATG AAGAGCAGCG CCCGGGTGGG CCTGGCGCGC
1201     GCTCGCACTC GCGGAGCGGA CCTCGGATGG GGAGAGACTG CGCAGGCGCA GTTCCCCGGG
1261     TGA

Quality Control:cDNA sequencing results is available upon request.