NAMPT / PBEF Protein (GST Tag)

Nicotinamide phosphoribosyltransferase Protein Datasheet

NAMPT / PBEF Protein Product Information

• Synonym: 1110035O14Rik, DKFZp666B131, MGC117256, PBEF, PBEF1, VF, VISFATIN
• Protein Construction: A DNA sequence encoding the human NAMPT (P43490) (Met 1-His 491 was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

NAMPT / PBEF Protein QC Testing

• Purity: >90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human NAMPT/GST chimera consists of 728 amino acids and has a calculated molecular mass of 83.3KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 8.0, 20%gly, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NAMPT / PBEF protein

NAMPT / PBEF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NAMPT / PBEF Protein Description

Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme belonging to the family of glycosyltransferases, to be specific, the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl- 1- pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD⁺ amounts in myeloid cells, as well as plasma NAMPT and NAD⁺ levels, were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.

References

1. Revollo JR. et al., 2007,. Cell Metab. 6 (5): 363-75.
2. Skokowa J. et al., 2009, Nat Med. 15 (2): 151-8.
3. Samal B. et al., 1994, Mol Cell Biol. 14 (2): 1431-7.