Human NAGA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13686-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

NAGA cDNA Clone Datasheet

NAGA cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000262.2
cDNA Size:	1236
Gene Synonym:	GALB; D22S674; NAGA
Source:	Human

NAGA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens N-acetylgalactosaminidase, alpha DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 279 G/A not causing the amino acid variation.

NAGA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NAGA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NAGA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCTGA AGACAGTGCT CTTGCTGGGA CATGTGGCCC AGGTGCTGAT GCTGGACAAT
61       GGGCTCCTGC AGACACCACC CATGGGCTGG CTGGCCTGGG AACGCTTCCG CTGCAACATT
121      AACTGTGATG AGGACCCAAA GAACTGCATA AGTGAACAGC TCTTCATGGA GATGGCTGAC
181      CGGATGGCAC AGGATGGATG GCGGGACATG GGCTACACAT ACCTCAACAT TGATGACTGC
241      TGGATCGGTG GTCGCGATGC CAGTGGCCGC CTGATGCCAG ATCCCAAGCG CTTCCCTCAT
301      GGCATTCCTT TCCTGGCTGA CTACGTTCAC TCCCTGGGCC TGAAGTTGGG TATCTACGCG
361      GACATGGGCA ACTTCACCTG CATGGGTTAC CCAGGCACCA CACTGGACAA GGTGGTCCAG
421      GATGCTCAGA CCTTCGCCGA GTGGAAGGTA GACATGCTCA AGCTGGATGG CTGCTTCTCC
481      ACCCCCGAGG AGCGGGCCCA GGGGTACCCC AAGATGGCTG CTGCCCTGAA TGCCACAGGC
541      CGCCCCATCG CCTTCTCCTG CAGCTGGCCA GCCTATGAAG GCGGCCTCCC CCCAAGGGTG
601      AACTACAGTC TGCTGGCGGA CATCTGCAAC CTCTGGCGTA ACTATGATGA CATCCAGGAC
661      TCCTGGTGGA GCGTGCTCTC CATCCTGAAT TGGTTCGTGG AGCACCAGGA CATACTGCAG
721      CCAGTGGCCG GCCCTGGGCA CTGGAATGAC CCTGACATGC TGCTCATTGG GAACTTTGGT
781      CTCAGCTTAG AGCAATCCCG GGCCCAGATG GCCCTGTGGA CGGTGCTGGC AGCCCCCCTC
841      TTGATGTCCA CAGACCTGCG TACCATCTCC GCCCAGAACA TGGACATTCT GCAGAATCCA
901      CTCATGATCA AAATCAACCA GGATCCCTTA GGCATCCAGG GACGCAGGAT TCACAAGGAA
961      AAATCTCTCA TCGAAGTGTA CATGCGGCCT CTGTCCAACA AGGCTAGCGC CTTAGTCTTC
1021     TTCAGCTGCA GGACCGATAT GCCTTATCGC TACCACTCCT CCCTTGGCCA GCTGAACTTC
1081     ACCGGGTCTG TGATATATGA GGCCCAGGAC GTCTACTCAG GTGACATCAT CAGTGGCCTC
1141     CGAGATGAAA CCAACTTCAC AGTGATCATC AACCCTTCAG GGGTAGTGAT GTGGTACCTG
1201     TATCCCATCA AGAACCTGGA GATGTCCCAG CAGTGA

Quality Control:cDNA sequencing results is available upon request.