Anti-N Cadherin Antibody

Cat: 11039-R020
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Anti-N Cadherin Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-N Cadherin Antibody
Validated applications
Species reactivity
Reacts with: Human
Human N Cadherin
Recombinant Human N-Cadherin / CD325 / CDH2 Protein (Catalog#11039-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; Catalog#11039-H08H; NP_001783.2; Met 1-Ala 724).
Monoclonal Rabbit IgG Clone #020
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-N Cadherin Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-N Cadherin Antibody (Rabbit Monoclonal antibody) Images
Human N-Cadherin/CD325/CDH2 Immunofluorescence(IF) 2962
Immunofluorescence staining of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to membrane.
Human N-Cadherin/CD325/CDH2 Flow Cytometry (FC) 4885
Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone R001 (upper), Clone R020 (bottom)] at matching concentrations , then a FITC-conjugated second step antibody.
Human N-Cadherin/CD325/CDH2 Western blot (WB) 13330

Anti-CDH2 rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:99 kDa

Observed band size:130 kDa

Anti-N Cadherin Antibody Alternative Names
Anti-CD325 Antibody;Anti-CDHN Antibody;Anti-CDw325 Antibody;Anti-NCAD Antibody
N Cadherin Background Information

Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.

Full Name
cadherin 2, type 1, N-cadherin (neuronal)
  • Jones M, et al. (2002) N-cadherin upregulation and function in response of smooth muscle cells to arterial injury. Arterioscler Thromb Vasc Biol. 22(12): 1972-7.
  • Nagi C, et al. (2005) N-cadherin expression in breast cancer: correlation with an aggressive histologic variant--invasive micropapillary carcinoma. Breast Cancer Res Treat. 94(3): 225-35.
  • Schrick C, et al. (2007) N-cadherin regulates cytoskeletally associated IQGAP1/ERK signaling and memory formation. Neuron. 55(5): 786-98.
  • Li K, et al. (2010) Downregulation of N-cadherin expression inhibits invasiveness, arrests cell cycle and induces cell apoptosis in esophageal squamous cell carcinoma. Cancer Invest. 28(5): 479-86.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-N Cadherin Antibody (FITC) Rabbit Monoclonal FCM 11039-R020-F
    Anti-N Cadherin Antibody (PE) Rabbit Monoclonal FCM 11039-R020-P
    Anti-N Cadherin Antibody (APC) Rabbit Monoclonal FCM 11039-R001-A
    Anti-N Cadherin Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 11039-R017
    Anti-N Cadherin Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 11039-R014
    Anti-N Cadherin Antibody Rabbit Monoclonal ELISA,FCM 11039-R001
    Anti-N Cadherin Antibody (APC) Rabbit Monoclonal FCM 11039-R020-A
    Anti-N Cadherin Antibody Rabbit Polyclonal ELISA 11039-RP01
    Anti-N Cadherin Antibody Rabbit Polyclonal ELISA 11039-T16
    Anti-N Cadherin Antibody (PE) Rabbit Monoclonal FCM 11039-R001-P
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