N-Cadherin / CD325 / CDH2 Antibody

N-Cadherin / CD325 / CDH2 Antibody Datasheet

N-Cadherin / CD325 / CDH2 Antibody Product Information

• Immunogen: Recombinant Human N-Cadherin / CD325 / CDH2 Protein (Catalog #11039-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 017
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; Catalog #11039-H08H; NP_001783.2; Met 1-Ala 724).

N-Cadherin / CD325 / CDH2 Antibody Usage Guide

• Specificity: Human N-Cadherin / CD325 / CDH2
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CDH2 in WB. Using a DAB detection system, the detection limit for Human CDH2 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CDH2. The detection limit for Human CDH2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

N-Cadherin / CD325 / CDH2 Antibody Background

Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Human cadherin 2, type 1, N-cadherin (neuronal) (NCAD) , also known as CDH2, is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-cadherin promoted apoptosis. During apoptosis, N-cadherin was cleaved into 20-100 kDa fragments. N-cadherin cleavage occurred at a higher level in glioblastoma cells than in non-neoplastic astrocytes.

References

1. LaMora A. et al., 2009, Neuroscience. 159:1175-1184.

2. Chauvet N. et al., 2009, J Endocrinol. 202:375-387.

3. Hartland S.N. et al., 2009, Liver Int. 29:966-978.

4. Kohutek Z.A. et al., 2009, J Neurosci. 29:4605-4615.