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Mouse VGF Gene ORF cDNA clone expression plasmid, C-His tag

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Mouse VGF cDNA Clone Product Information
NCBI RefSeq:NM_001039385.1
RefSeq ORF Size:1854bp
cDNA Description:Full length Clone DNA of Mus musculus VGF nerve growth factor inducible with C terminal His tag.
Gene Synonym:Gm1052
Species:Mouse
Vector:pCMV3-C-His
Plasmid:pCMV3-mVGF-His
Restriction Site:KpnI + XbaI (6kb + 1.90kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Mouse VGF Gene Plasmid Map
Mouse VGF natural ORF mammalian expression plasmid, C-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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  • Mouse VGF natural ORF mammalian expression plasmid, C-His tag
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