|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG52988-ACG|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG52988-ACR|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, C-Flag tag||MG52988-CF|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, C-His tag||MG52988-CH|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, C-Myc tag||MG52988-CM|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, C-HA tag||MG52988-CY|
|Mouse UBE2I Gene ORF cDNA clone in cloning vector||MG52988-G|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, N-Flag tag||MG52988-NF|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, N-His tag||MG52988-NH|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, N-Myc tag||MG52988-NM|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid, N-HA tag||MG52988-NY|
|Mouse UBE2I Gene ORF cDNA clone expression plasmid||MG52988-UT|
|Learn more about expression Vectors|
UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.