Mouse SPOCK3 Baculovirus-Insect Overexpression Lysate: Product Information
This Mouse SPOCK3 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of SPOCK3 protein (Cat: 50459-M08B) from the overexpression lysate was verified.
A DNA sequence encoding the mouse Spock3 (NP_076178.1) (Met1-Ile436) was expressed with a polyhistidine tag at the C-terminus.
The recombinant mouse Spock3 consists 425 amino acids and predicts a molecular mass of 48.3 kDa.
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
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