Thy1 qPCR Primer Pairs, Mouse

Cat: MP200076
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Thy1 qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Thy1 qPCR Primer Pairs, Mouse Alternative Names
CD90 qPCR Primer Pairs, Mouse;T25 qPCR Primer Pairs, Mouse;Thy-1 qPCR Primer Pairs, Mouse;Thy-1.2 qPCR Primer Pairs, Mouse;Thy1.1 qPCR Primer Pairs, Mouse;Thy1.2 qPCR Primer Pairs, Mouse
Thy1 Background Information

Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.

Full Name
Thy-1 cell surface antigen
References
  • Rege TA, et al. (2006) Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 20(8): 1045-54.
  • Fiegel HC, et al. (2008) Lack of Thy1 (CD90) expression in neuroblastomas is correlated with impaired survival. Pediatr Surg Int. 24(1): 101-5.
  • Bradley JE, et al. (2009) Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 35(3): 258-65.
  • Kisselbach L, et al. (2009) CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures. Cytotechnology. 59(1): 31-44.
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    Product Name Catalog#(PDF)
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    Thy1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80337-CF
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    Thy1 cDNA ORF Clone, Rat, C-His tag RG80337-CH
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    Thy1 cDNA ORF Clone, Rat, N-HA tag RG80337-NY
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    Product Name Catalog#(PDF)
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    Thy1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50461-ACG
    Thy1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50461-ACR
    Thy1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50461-CF
    Thy1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50461-NF
    Thy1 cDNA ORF Clone, Mouse, C-His tag MG50461-CH
    Thy1 cDNA ORF Clone, Mouse, N-His tag MG50461-NH
    Thy1 cDNA ORF Clone, Mouse, C-HA tag MG50461-CY
    Thy1 cDNA ORF Clone, Mouse, N-HA tag MG50461-NY
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    Product Name Catalog#(PDF)
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    Thy1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG16897-ACGLN
    Thy1 cDNA ORF Clone, Human, C-OFPSpark® tag HG16897-ACR
    Thy1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG16897-CF
    Thy1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG16897-NF
    Thy1 cDNA ORF Clone, Human, C-His tag HG16897-CH
    Thy1 cDNA ORF Clone, Human, N-His tag HG16897-NH
    Thy1 cDNA ORF Clone, Human, C-HA tag HG16897-CY
    Thy1 cDNA ORF Clone, Human, N-HA tag HG16897-NY
    Thy1 cDNA ORF Clone, Human, C-Myc tag HG16897-CM
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