SLIT2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag General Information
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 819C/T, 1512T/C, 1716C/T, 2217T/C and 3610A/C not causing the amino acid variation.
Full length Clone DNA of Mouse slit homolog 2 (Drosophila) with C terminal Flag tag.
Enhanced CMV promoter
KpnI + XbaI (6kb + 4.62kb)
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
SLIT2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
SLIT2 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names
Drad-1 cDNA ORF Clone, Mouse;E030015M03Rik cDNA ORF Clone, Mouse;E130320P19Rik cDNA ORF Clone, Mouse;mKIAA4141 cDNA ORF Clone, Mouse;Slil3 cDNA ORF Clone, Mouse;slit-2 cDNA ORF Clone, Mouse
SLIT2 Background Information
slit guidance ligand 2