SIRT5 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag

Cat: MG50483-CF
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SIRT5 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag General Information
Gene
Species
Mouse
NCBI Ref Seq
RefSeq ORF Size
933 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutation: 6 A/T not causing the amino acid variation.
Description
Full length Clone DNA of Mouse sirtuin 5 (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + XbaI (6kb + 0.98kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
SIRT5 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SIRT5 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Validated Images
Mouse SIRT5 Gene Expression validated Image 2189
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609
SIRT5 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag Alternative Names
0610012J09Rik cDNA ORF Clone, Mouse;1500032M05Rik cDNA ORF Clone, Mouse;AV001953 cDNA ORF Clone, Mouse
SIRT5 Background Information
Full Name
sirtuin 5

SIRT5 cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
SIRT5 cDNA ORF Clone in Cloning Vector, Rat RG81260-U
SIRT5 cDNA ORF Clone, Rat, untagged RG81260-UT
SIRT5 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81260-ACGLN
SIRT5 cDNA ORF Clone, Rat, C-GFPSpark® tag RG81260-ACG
SIRT5 cDNA ORF Clone, Rat, N-GFPSpark® tag RG81260-ANG
SIRT5 cDNA ORF Clone, Rat, C-OFPSpark® tag RG81260-ACR
SIRT5 cDNA ORF Clone, Rat, N-OFPSpark® tag RG81260-ANR
SIRT5 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG81260-CF
SIRT5 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG81260-NF
SIRT5 cDNA ORF Clone, Rat, C-His tag RG81260-CH
SIRT5 cDNA ORF Clone, Rat, N-His tag RG81260-NH
SIRT5 cDNA ORF Clone, Rat, C-HA tag RG81260-CY
SIRT5 cDNA ORF Clone, Rat, N-HA tag RG81260-NY
SIRT5 cDNA ORF Clone, Rat, C-Myc tag RG81260-CM
SIRT5 cDNA ORF Clone, Rat, N-Myc tag RG81260-NM
SIRT5 qPCR Primer Pairs, Rat RP301216

SIRT5 cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
SIRT5 cDNA ORF Clone in Cloning Vector, Mouse MG50483-M
SIRT5 cDNA ORF Clone, Mouse, untagged MG50483-UT
SIRT5 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50483-ACGLN
SIRT5 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50483-ACG
SIRT5 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50483-ANG
SIRT5 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50483-ACR
SIRT5 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50483-ANR
SIRT5 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50483-NF
SIRT5 cDNA ORF Clone, Mouse, C-His tag MG50483-CH
SIRT5 cDNA ORF Clone, Mouse, N-His tag MG50483-NH
SIRT5 cDNA ORF Clone, Mouse, C-HA tag MG50483-CY
SIRT5 cDNA ORF Clone, Mouse, N-HA tag MG50483-NY
SIRT5 cDNA ORF Clone, Mouse, C-Myc tag MG50483-CM
SIRT5 cDNA ORF Clone, Mouse, N-Myc tag MG50483-NM
SIRT5 qPCR Primer Pairs, Mouse MP200477

SIRT5 cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
SIRT5 cDNA ORF Clone, Human, untagged HG19202-UT
SIRT5 cDNA ORF Clone, Human, C-His tag HG19202-CH
info info
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