|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse SIRPB1A ORF mammalian expression plasmid, C-GFPSpark tag||MG50816-ACG|
|Mouse SIRPB1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50816-ACR|
|Mouse SIRPB1A ORF mammalian expression plasmid, C-Flag tag||MG50816-CF|
|Mouse SIRPB1A ORF mammalian expression plasmid, C-His tag||MG50816-CH|
|Mouse SIRPB1A ORF mammalian expression plasmid, C-Myc tag||MG50816-CM|
|Mouse SIRPB1A ORF mammalian expression plasmid, C-HA tag||MG50816-CY|
|Mouse SIRPB1A Gene cDNA clone plasmid||MG50816-G|
|Mouse SIRPB1A ORF mammalian expression plasmid, N-Flag tag||MG50816-NF|
|Mouse SIRPB1A ORF mammalian expression plasmid, N-His tag||MG50816-NH|
|Mouse SIRPB1A ORF mammalian expression plasmid, N-Myc tag||MG50816-NM|
|Mouse SIRPB1A ORF mammalian expression plasmid, N-HA tag||MG50816-NY|
|Mouse SIRPB1A natural ORF mammalian expression plasmid||MG50816-UT|
|Learn more about expression Vectors|
SIRPB1A (Signal-regulatory protein beta 1A), also known as SIRP beta 1, belongs to signal-regulatory-protein (SIRP) family, and immunoglobulin superfamily. Signal-regulatory proteins (SIRPs) are cell-surface glycoproteins expressed on myeloid and neural cells that have been shown to recruit SH2 domain-containing protein phosphatase 1 (SHP-1) and SHP-2 and to regulate receptor tyrosine kinase-coupled signaling. SIRP are classified as SIRP alpha molecules, containing a 110- to 113-amino acid long, or SIRP beta molecules, with a 5-amino acid long intracytoplasmic domain. SIRP beta 1 is a new DAP12-associated receptor involved in the activation of myeloid cells, which contains a short cytoplasmic domain that lacks sequence motifs capable of recruiting SHP-1 and SHP-2. SIRP beta 1. SIRP beta 1 acts as an activating isoform of SIRP alpha molecules, confirming the co-existence of inhibitory ITIM-bearing molecules, recruiting SHP-1 and SHP-2 protein tyrosine phosphatases, and activating counterparts, whose engagement couples to protein tyrosine kinases via ITAM-bearing molecules.