|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse SIAE ORF mammalian expression plasmid, C-GFPSpark tag||MG53030-ACG|
|Mouse SIAE ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG53030-ACR|
|Mouse SIAE ORF mammalian expression plasmid, C-Flag tag||MG53030-CF|
|Mouse SIAE ORF mammalian expression plasmid, C-His tag||MG53030-CH|
|Mouse SIAE ORF mammalian expression plasmid, C-Myc tag||MG53030-CM|
|Mouse SIAE ORF mammalian expression plasmid, C-HA tag||MG53030-CY|
|Mouse SIAE Gene cDNA clone plasmid||MG53030-G|
|Mouse SIAE ORF mammalian expression plasmid, N-Flag tag||MG53030-NF|
|Mouse SIAE ORF mammalian expression plasmid, N-His tag||MG53030-NH|
|Mouse SIAE ORF mammalian expression plasmid, N-Myc tag||MG53030-NM|
|Mouse SIAE ORF mammalian expression plasmid, N-HA tag||MG53030-NY|
|Mouse SIAE natural ORF mammalian expression plasmid||MG53030-UT|
|Learn more about expression Vectors|
Sialate O-acetylesterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. It is widely expressed with high expression in the testis, prostate, and colon. The systematic name of this enzyme class is N-acyl-O-acetylneuraminate O-acetylhydrolase. Other names in common use include N-acetylneuraminate acetyltransferase, sialate 9(4)-O-acetylesterase, and sialidase. Sialate O-acetylesterase catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. Defects in Sialate O-acetylesterase are a cause of autoimmune disease type 6 (AIS6). Individuals manifesting susceptibility to autoimmune disease type 6 can suffer from juvenile idiopathic arthritis, rheumatoid arthritis, multiple sclerosis, Sjogren syndrome, systemic lupus erythematosus, type 1 diabetes, ulcerative colitis, and Crohn disease.