|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse PLA2G1B ORF mammalian expression plasmid, C-GFPSpark tag||MG50329-ACG|
|Mouse PLA2G1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50329-ACR|
|Mouse PLA2G1B ORF mammalian expression plasmid, C-Flag tag||MG50329-CF|
|Mouse PLA2G1B ORF mammalian expression plasmid, C-His tag||MG50329-CH|
|Mouse PLA2G1B ORF mammalian expression plasmid, C-Myc tag||MG50329-CM|
|Mouse PLA2G1B ORF mammalian expression plasmid, C-HA tag||MG50329-CY|
|Mouse PLA2G1B Gene cDNA clone plasmid||MG50329-M|
|Mouse PLA2G1B ORF mammalian expression plasmid, N-Flag tag||MG50329-NF|
|Mouse PLA2G1B ORF mammalian expression plasmid, N-His tag||MG50329-NH|
|Mouse PLA2G1B ORF mammalian expression plasmid, N-Myc tag||MG50329-NM|
|Mouse PLA2G1B ORF mammalian expression plasmid, N-HA tag||MG50329-NY|
|Mouse PLA2G1B natural ORF mammalian expression plasmid||MG50329-UT|
|Learn more about expression Vectors|
Mouse phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.