|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse p53 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||MG50534-ACG|
|Mouse p53 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||MG50534-ACR|
|Mouse p53 Gene ORF cDNA clone expression plasmid, N-GFPSpark tag||MG50534-ANG|
|Mouse p53 Gene ORF cDNA clone expression plasmid, N-OFPSpark tag||MG50534-ANR|
|Mouse p53 Gene ORF cDNA clone expression plasmid, C-Flag tag||MG50534-CF|
|Mouse p53 Gene ORF cDNA clone expression plasmid, C-His tag||MG50534-CH|
|Mouse p53 Gene ORF cDNA clone expression plasmid, C-Myc tag||MG50534-CM|
|Mouse p53 Gene ORF cDNA clone expression plasmid, C-HA tag||MG50534-CY|
|Mouse p53 Gene ORF cDNA clone in cloning vector||MG50534-M|
|Mouse p53 Gene ORF cDNA clone expression plasmid, N-Flag tag||MG50534-NF|
|Mouse p53 Gene ORF cDNA clone expression plasmid, N-His tag||MG50534-NH|
|Mouse p53 Gene ORF cDNA clone expression plasmid, N-Myc tag||MG50534-NM|
|Mouse p53 Gene ORF cDNA clone expression plasmid, N-HA tag||MG50534-NY|
|Mouse p53 Gene ORF cDNA clone expression plasmid||MG50534-UT|
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p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.