Mouse NOTCH1 Baculovirus-Insect cells Overexpression Lysate

Cat: 50654-M08BL
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Mouse NOTCH1 Baculovirus-Insect cells Overexpression Lysate Product Information
Product Description
This Mouse NOTCH1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of NOTCH1 protein (Cat: 50654-M08B) from the overexpression lysate was verified.
Expression Host
Baculovirus-Insect cells
Species
Mouse
Sequence Information
A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed,with a C-terminal polyhistidine tag.
Molecule Mass
The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
Mouse NOTCH1 Baculovirus-Insect cells Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Mouse NOTCH1 Baculovirus-Insect cells Overexpression Lysate Alternative Names
Mouse 9930111A19Rik Overexpression Lysate;Mouse lin-12 Overexpression Lysate;Mouse Mis6 Overexpression Lysate;Mouse N1 Overexpression Lysate;Mouse Tan1 Overexpression Lysate
NOTCH1 Background Information
Full Name
notch 1

Mouse Overexpression Lysate

Product Name Cat.No.
Mouse NOTCH1 CHO Stable Overexpression Lysate 50654-M02SL

Human Overexpression Lysate

Product Name Cat.No.
Human NOTCH1 HEK293 Overexpression Lysate 10954-H02HL
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