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Mouse Notch-1 / NOTCH1 Insect Cell Lysate (WB positive control)

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    Mouse Notch-1 Transfected / Overexpression Cell Lysate Product Information
    Expressed Host:Baculovirus-Insect cells
    Product Description:Baculovirus-Insect Cell lysate that Mouse NOTCH1 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
    Sequence information:A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed,with a C-terminal polyhistidine tag.
    Predicted N Terminal:Ala 19
    Molecule Mass:The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
    Species:Mouse
    Mouse Notch-1 Transfected / Overexpression Cell Lysate Usage Guide
    Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
    Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
    Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
    Stability:Samples are stable for up to twelve months from date of receipt.
    Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
    Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
    Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
    Application notes:Western blot (WB): Use at an assay dependent dilution.
    Other Applications: Not tested.
    Optimal dilutions/concentrations should be determined by the end user.
    Size / Price
    Catalog: 50654-M08BL-300
    List Price: 
    Price:      (You Save: )
    Size:
    300 µg
    Quantity:+-
    Availability2 weeks
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"