Mouse Monoclonal Antibody Full Service Platform
Sino Biological Inc.
Your Centralized Protein / Antibody Development / Production Lab
Attractive Cost Saving for Products & Services
Sino Biological has optimized the entire mouse monoclonal antibody development process to increase efficiency and probability of success. We have developed and optimized various methods for antigen conjugation and adjuvant design, immunization methods and schedule, hybridoma fusion technology, antibody screening methods, and hybridoma cell culture processes. We can use either recombinant protein as antigen or conjugated peptide as antigen for immunization.
All of our mouse monoclonal antibodies were produced using our advanced hybridoma cell culture technologies. We do not use mice for antibody production. For difficult-to-produce mouse monoclonal antibodies, we have the experience and expertise to clone the mouse antibody genes out and insert them,into our transient transfection vectors for more efficient recombinant production in either HEK293 or CHO cells. We have extensive experience and expertise in purifying all types of mouse antibodies including IgG1, IgG2a, IgG2b, IgG3, and IgM.
By using recombinant protein based antigen for immunization, Sino Biological can obtain high-affinity mouse monoclonal antibodies for ELISA assay as well as western Blotting, IHC, and flow cytometry applications. Sino Biological has successfully developed ELISA assays with mouse monoclonal antibodies with pg assay sensitivity. Our mouse monoclonal antibodies usually have high binding affinity and low detection limits in the range of 1-10 ng/well.