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Mouse Matriptase/ST14 Gene ORF cDNA clone expression plasmid, C-His tag

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Mouse ST14 cDNA Clone Product Information
NCBI RefSeq:NM_011176.4
RefSeq ORF Size:2568bp
cDNA Description:Full length Clone DNA of Mus musculus suppression of tumorigenicity 14 (colon carcinoma) with C terminal His tag.
Gene Synonym:MT-SP1, Prss14, Epithin, matriptase, St14
Species:Mouse
Vector:pCMV3-C-His
Plasmid:pCMV3-mST14-His
Restriction Site:KpnI(two restriction sites) + XbaI (6kb + 2.27kb + 0.35kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Mouse ST14 Gene Plasmid Map
Mouse Matriptase / ST14 ORF mammalian expression plasmid, C-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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