MIF qPCR Primer Pairs, Mouse

Cat: MP200066
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MIF qPCR Primer Pairs, Mouse General Information
Target Details
Species:
Mouse
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
MIF qPCR Primer Pairs, Mouse Alternative Names
GIF qPCR Primer Pairs, Mouse;Glif qPCR Primer Pairs, Mouse
MIF Background Information

Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is an ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important roles in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.

Full Name
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
References
  • Ohkawara T, et al. (2005) Pathophysiological roles of macrophage migration inhibitory factor in gastrointestinal, hepatic, and pancreatic disorders. J Gastroenterol. 40(2): 117-22.
  • Bach JP, et al.. (2009) The role of macrophage inhibitory factor in tumorigenesis and central nervous system tumors. Cancer. 115(10): 2031-40.
  • Rendon BE, et al.. (2009) Mechanisms of macrophage migration inhibitory factor (MIF)-dependent tumor microenvironmental adaptation. Exp Mol Pathol. 86(3): 180-5.
  • Grieb G, et al. (2010) Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News Perspect. 23(4): 257-64.
  • MIF qPCR Primer Pairs, Mouse, Related Products

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    MIF cDNA ORF Clone in Cloning Vector, Mouse MG50066-M
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    MIF cDNA ORF Clone, Mouse, C-His tag MG50066-CH
    MIF cDNA ORF Clone, Mouse, N-His tag MG50066-NH
    MIF cDNA ORF Clone, Mouse, C-HA tag MG50066-CY
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    MIF qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    MIF cDNA ORF Clone in Cloning Vector, Human HG10246-M
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    MIF cDNA ORF Clone, Human, C-GFPSpark® tag HG10246-ACG
    MIF cDNA ORF Clone, Human, C-OFPSpark® tag HG10246-ACR
    MIF cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10246-CF
    MIF cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10246-NF
    MIF cDNA ORF Clone, Human, C-His tag HG10246-CH
    MIF cDNA ORF Clone, Human, N-His tag HG10246-NH
    MIF cDNA ORF Clone, Human, C-HA tag HG10246-CY
    MIF cDNA ORF Clone, Human, N-HA tag HG10246-NY
    MIF cDNA ORF Clone, Human, C-Myc tag HG10246-CM
    MIF cDNA ORF Clone, Human, N-Myc tag HG10246-NM
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