|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse MEP1A ORF mammalian expression plasmid, C-GFPSpark tag||MG50057-ACG|
|Mouse MEP1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50057-ACR|
|Mouse MEP1A ORF mammalian expression plasmid, C-Flag tag||MG50057-CF|
|Mouse MEP1A ORF mammalian expression plasmid, C-His tag||MG50057-CH|
|Mouse MEP1A ORF mammalian expression plasmid, C-Myc tag||MG50057-CM|
|Mouse MEP1A ORF mammalian expression plasmid, C-HA tag||MG50057-CY|
|Mouse MEP1A Gene cDNA clone plasmid||MG50057-M|
|Mouse MEP1A ORF mammalian expression plasmid, N-Flag tag||MG50057-NF|
|Mouse MEP1A ORF mammalian expression plasmid, N-His tag||MG50057-NH|
|Mouse MEP1A ORF mammalian expression plasmid, N-Myc tag||MG50057-NM|
|Mouse MEP1A ORF mammalian expression plasmid, N-HA tag||MG50057-NY|
|Mouse MEP1A natural ORF mammalian expression plasmid||MG50057-UT|
|Learn more about expression Vectors|
Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).